250 kDa
130 kDa
100 kDa
70 kDa
55 kDa
35 kDa
C o n t r o l F L A G
- T A N
C
1
F L A G
- T A N
C
1
( - A A A )
B)
A)
TANC1
TANC1-AAA
TANC1
AGAP3
C10
SET
TRIM41
AGAP1
ANP32A
NFATC3
SCRIB
DLG1
MPP7
CASK
PTPN13
SNX27
DDB1
MAP1B
HADHA
APC
VPRBP
USP9X
CTNNB1
TNIK
APPBP2
SPECC1L
HADHB
PPP6R3
CTNNA1
PJA2
KCTD5
COPA
PHGDH
MINK1
AKAP11
SDCCAG3
HTATSF1
CPVL
FAM190B
PRKACA
RASAL2
PPP1R12A
SNX9
USP7
SNX33
POLR2B
ANP32B
GTF2I
NACA
SDC2
PPP2R2A
HIST1H2BC
AMOT
FKBP3
CTNND1
MSH6
Fig. 3 Identification of FLAG-TANC1 partners by immunoprecipitation. (a)
SDS-PAGE separation of protein complexes associated to FLAG-TANC1 purified
by immunoprecipitation. Purified protein complexes associated to wild type
FLAG-TANC1 (left lane) or its mutant form (right lane) were separated on
4–12% NuPAGE
®
acrylamide gel and silver stained. (b) List of proteins associated to TANC1 through its PDZ binding motif. PDZ domain proteins are in red
24
Avais M. Daulat et al.