References
1. Fields S, Song O (1989) A novel genetic system
to detect protein–protein interactions. Nature
340:245–246
2. Bru ¨ckner A, Polge C, Lentze N, Auerbach D,
Schlattner U (2009) Yeast two-hybrid, a powerful tool for systems biology. Int J Mol Sci
10:2763–2788
3. Zhu ZX, Yu ZM, Taylor JL, Wu YH, Ni J
(2016) The application of yeast hybrid systems
in protein interaction analysis. Mol Biol
50:663–670
4. Rodrı ´guez-Negrete E, Bejarano ER, Castillo
AG (2014) Using the yeast two-hybrid system
to identify protein–protein interactions. In:
Jorrin-Novo JV, Komatsu S, Weckwerth W
et al (eds) Plant proteomics: methods and protocols. Humana Press, Totowa, NJ, pp
241–258
5. Fields S (2005) High-throughput two-hybrid
analysis. The promise and the peril. FEBS J
272:5391–5399
6. H€ auser R, Stellberger T, Rajagopala SV, Uetz P
(2012) Matrix-based yeast two-hybrid screen
strategies and comparison of systems. Methods
Mol Biol 812:1–20
7. Galletta BJ, Rusan NM (2015) A yeast
two-hybrid
approach
for
probing
protein–protein interactions at the centrosome. Methods Cell Biol 129:251–277
8. Lenfant N, Polanowska J, Bamps S, Omi S,
Borg JP, Reboul J (2010) A genome-wide
study of PDZ-domain interactions in
C. elegans reveals a high frequency of
non-canonical binding. BMC Genomics
11:671
9. Rajagopala SV, Sikorski P, Caufield JH,
Tovchigrechko A, Uetz P (2012) Studying
protein complexes by the yeast two-hybrid system. Methods 58:392–399
10. Luck K, Charbonnier S, Trave ´ G (2012) The
emerging contribution of sequence context to
the specificity of protein interactions mediated
by PDZ domains. FEBS Lett 586:2648–2661
11. Songyang Z (1997) Recognition of unique
carboxyl-terminal motifs by distinct PDZ
domains. Science 275:73–77
12. Belotti E, Polanowska J, Daulat A, Audebert S,
Thome ´ V, Lissitzky J-C, Lembo F, Blibek K,
Omi S, Lenfant N, Gangar A, Montcouquiol
M, Santoni M-J, Sebbagh M, Aurrand-Lions
M, Angers S, Kodjabachian L, Reboul J, Borg
J-P (2013) The Human PDZome: A Gateway
to PSD95-Disc Large-Zonula Occludens
(PDZ)-mediated Functions. Mol Cell Proteomics 12:2587–2603
Y2H Screening of PDZ Interactions
15
1. Fields S, Song O (1989) A novel genetic system
to detect protein–protein interactions. Nature
340:245–246
2. Bru ¨ckner A, Polge C, Lentze N, Auerbach D,
Schlattner U (2009) Yeast two-hybrid, a powerful tool for systems biology. Int J Mol Sci
10:2763–2788
3. Zhu ZX, Yu ZM, Taylor JL, Wu YH, Ni J
(2016) The application of yeast hybrid systems
in protein interaction analysis. Mol Biol
50:663–670
4. Rodrı ´guez-Negrete E, Bejarano ER, Castillo
AG (2014) Using the yeast two-hybrid system
to identify protein–protein interactions. In:
Jorrin-Novo JV, Komatsu S, Weckwerth W
et al (eds) Plant proteomics: methods and protocols. Humana Press, Totowa, NJ, pp
241–258
5. Fields S (2005) High-throughput two-hybrid
analysis. The promise and the peril. FEBS J
272:5391–5399
6. H€ auser R, Stellberger T, Rajagopala SV, Uetz P
(2012) Matrix-based yeast two-hybrid screen
strategies and comparison of systems. Methods
Mol Biol 812:1–20
7. Galletta BJ, Rusan NM (2015) A yeast
two-hybrid
approach
for
probing
protein–protein interactions at the centrosome. Methods Cell Biol 129:251–277
8. Lenfant N, Polanowska J, Bamps S, Omi S,
Borg JP, Reboul J (2010) A genome-wide
study of PDZ-domain interactions in
C. elegans reveals a high frequency of
non-canonical binding. BMC Genomics
11:671
9. Rajagopala SV, Sikorski P, Caufield JH,
Tovchigrechko A, Uetz P (2012) Studying
protein complexes by the yeast two-hybrid system. Methods 58:392–399
10. Luck K, Charbonnier S, Trave ´ G (2012) The
emerging contribution of sequence context to
the specificity of protein interactions mediated
by PDZ domains. FEBS Lett 586:2648–2661
11. Songyang Z (1997) Recognition of unique
carboxyl-terminal motifs by distinct PDZ
domains. Science 275:73–77
12. Belotti E, Polanowska J, Daulat A, Audebert S,
Thome ´ V, Lissitzky J-C, Lembo F, Blibek K,
Omi S, Lenfant N, Gangar A, Montcouquiol
M, Santoni M-J, Sebbagh M, Aurrand-Lions
M, Angers S, Kodjabachian L, Reboul J, Borg
J-P (2013) The Human PDZome: A Gateway
to PSD95-Disc Large-Zonula Occludens
(PDZ)-mediated Functions. Mol Cell Proteomics 12:2587–2603
Y2H Screening of PDZ Interactions
15