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2 interacts with and phosphorylates PDZ
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high-throughput fluorescence polarization
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Fluorescence polarization assays in highthroughput screening and drug discovery: a
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23. Roehrl MHA, Wang JY, Wagner G (2004) A
general framework for development and data
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screens for small-molecule inhibitors of
protein-protein interactions by fluorescence
polarization. Biochemistry 43:16056–16066.
https://doi.org/10.1021/bi048233g
24. Macek P, Cliff MJ, Embrey KJ et al (2018) Myc
phosphorylation in its basic helix-loop-helix
region destabilizes transient-helical structures,
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25. Dixon AS, Schwinn MK, Hall MP et al (2016)
NanoLuc complementation reporter optimized for accurate measurement of protein
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11:400–408.
https://doi.org/10.1021/
acschembio.5b00753
26. Simon M, Go ´ gl G, Ecse ´di P, et al (2019) High
throughput competitive fluorescence polarization assay reveals functional redundancy in the
S100 protein family. bioRxiv. https://doi.org/
10.1101/718155
192
Ma ´ rton A. Simon and La ´ szlo ´ Nyitray
Huganir RL (2005) Ribosomal S6 kinase
2 interacts with and phosphorylates PDZ
domain-containing proteins and regulates
AMPA receptor transmission. Proc Natl Acad
Sci U S A 102:15006–15011. https://doi.
org/10.1073/pnas.0507476102
19. Shi GX, Yang WS, Jin L et al (2017) RSK2
drives cell motility by serine phosphorylation
of LARG and activation of rho GTPases. Proc
Natl Acad Sci U S A 115:E190–E199. https://
doi.org/10.1073/pnas.1708584115
20. Park S-H, Raines RT (2015) Fluorescence
polarization assay to quantify protein-protein
interactions.
Methods
Mol
Biol
261:161–165. https://doi.org/10.1385/159259-762-9:161
21. Roehrl MHA, Wang JY, Wagner G (2004)
Discovery of small-molecule inhibitors of the
NFAT-calcineurin interaction by competitive
high-throughput fluorescence polarization
screening. Biochemistry 43:16067–16075.
https://doi.org/10.1021/bi048232o
22. Hall MD, Yasgar A, Peryea T et al (2016)
Fluorescence polarization assays in highthroughput screening and drug discovery: a
review. Methods Appl Fluoresc 4:022001.
https://doi.org/10.1088/2050-6120/4/2/
022001
23. Roehrl MHA, Wang JY, Wagner G (2004) A
general framework for development and data
analysis of competitive high-throughput
screens for small-molecule inhibitors of
protein-protein interactions by fluorescence
polarization. Biochemistry 43:16056–16066.
https://doi.org/10.1021/bi048233g
24. Macek P, Cliff MJ, Embrey KJ et al (2018) Myc
phosphorylation in its basic helix-loop-helix
region destabilizes transient-helical structures,
disrupting max and DNA binding. J Biol Chem
293:9301–9310. https://doi.org/10.1074/
jbc.RA118.002709
25. Dixon AS, Schwinn MK, Hall MP et al (2016)
NanoLuc complementation reporter optimized for accurate measurement of protein
interactions in cells. ACS Chem Biol
11:400–408.
https://doi.org/10.1021/
acschembio.5b00753
26. Simon M, Go ´ gl G, Ecse ´di P, et al (2019) High
throughput competitive fluorescence polarization assay reveals functional redundancy in the
S100 protein family. bioRxiv. https://doi.org/
10.1101/718155
192
Ma ´ rton A. Simon and La ´ szlo ´ Nyitray