prepare a complex of an individual PDZ domain with a peptide
corresponding to the C-terminal PDZ binding motif (PBM) of the
interacting partner that has been identified using ITC. In general
PDZ domains typically harbor micromolar affinities toward their
binding partners, and the outlined method has been successfully
used to prepare complexes for crystallization trials of Scribble/Dlg
PDZ domains with peptides [25, 26] and led to the determination
of a number of crystal structures (see Table 1 and Fig. 1).
Fig. 1 Biophysical characterization of Scribble PDZ domain–ligand complexes. (a) Cartoon structure of D.
melanogaster Scribble PDZ1 domain bound to Gukh with associated interaction measured by ITC and CD
spectrum of Scribble PDZ1 domain. (b) Cartoon structure of human Scribble PDZ1 domain bound to phosphoMCC with associated interaction measured by ITC and CD spectrum of Scribble PDZ1 domain. (c) Cartoon
structure of human Scribble PDZ3 domain bound to β-Pix with associated interaction measured by ITC and CD
spectrum of Scribble PDZ3 domain
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