19. The use of an incubator with a clear glass window/door is a
recommended biosafety containment feature because a malfunction associated with the HFC or duet pump can be seen
before the incubator door is opened and an appropriate remediation procedure initiated.
20. From a biosafety perspective, inoculation of the HFC and
removal of ECS samples are the most hazardous procedures
performed given the potentially high numbers of bacteria
being manipulated. Both operations are performed inside the
BSC to minimize the risk of spills and contain any aerosols that
might be generated.
21. Additional guidelines for the use of HF model of tuberculosis
can be found in the HFS-TB laboratory manual [5].
Acknowledgements
This work was supported by grant OPP1154895 from the Bill and
Melinda Gates Foundation.
References
1. Zinner SH, Husson M, Klastersky J (1981) An
artificial capillary in vitro kinetic model of antibiotic bactericidal activity. J Infect Dis
144:583–587
2. Gumbo T, Louie A, Deziel MR et al (2004)
Selection of a moxifloxacin dose that suppresses
drug resistance in Mycobacterium tuberculosis, by
use of an in vitro pharmacodynamics infection
model and mathematical modeling. J Infect Dis
190:1642–1651
3. Deshpande D, Srivastava S, Meek C et al (2010)
Ethambutol optimal clinical dose and susceptibility breakpoint identification by use of a novel
pharmacokinetic-pharmacodynamic model of
disseminated
intracellular
Mycobacterium
avium. Antimicrob Agents Chemother 54
(5):1728–1733
4. Srivastava S, Pasipanodya JG, Ramachandran G
et al (2016) A long-term co-perfused
disseminated tuberculosis-3D liver hollow fiber
model for both drug efficacy and hepatotoxicity
in babies. EBioMedicine 6:126–138
5. Hollow Fiber System of Tuberculosis (HFS-TB)
A laboratory manual to guide system engineering, study design and execution version 1.0 June
2018 (Critical Path Institute https://c-path.
research-readiness-tools/hollow-fiber-systemof-tuberculosis-laboratory-manual/)
Mycobacterium Tuberculosis in a Hollow Fiber System
731
recommended biosafety containment feature because a malfunction associated with the HFC or duet pump can be seen
before the incubator door is opened and an appropriate remediation procedure initiated.
20. From a biosafety perspective, inoculation of the HFC and
removal of ECS samples are the most hazardous procedures
performed given the potentially high numbers of bacteria
being manipulated. Both operations are performed inside the
BSC to minimize the risk of spills and contain any aerosols that
might be generated.
21. Additional guidelines for the use of HF model of tuberculosis
can be found in the HFS-TB laboratory manual [5].
Acknowledgements
This work was supported by grant OPP1154895 from the Bill and
Melinda Gates Foundation.
References
1. Zinner SH, Husson M, Klastersky J (1981) An
artificial capillary in vitro kinetic model of antibiotic bactericidal activity. J Infect Dis
144:583–587
2. Gumbo T, Louie A, Deziel MR et al (2004)
Selection of a moxifloxacin dose that suppresses
drug resistance in Mycobacterium tuberculosis, by
use of an in vitro pharmacodynamics infection
model and mathematical modeling. J Infect Dis
190:1642–1651
3. Deshpande D, Srivastava S, Meek C et al (2010)
Ethambutol optimal clinical dose and susceptibility breakpoint identification by use of a novel
pharmacokinetic-pharmacodynamic model of
disseminated
intracellular
Mycobacterium
avium. Antimicrob Agents Chemother 54
(5):1728–1733
4. Srivastava S, Pasipanodya JG, Ramachandran G
et al (2016) A long-term co-perfused
disseminated tuberculosis-3D liver hollow fiber
model for both drug efficacy and hepatotoxicity
in babies. EBioMedicine 6:126–138
5. Hollow Fiber System of Tuberculosis (HFS-TB)
A laboratory manual to guide system engineering, study design and execution version 1.0 June
2018 (Critical Path Institute https://c-path.
research-readiness-tools/hollow-fiber-systemof-tuberculosis-laboratory-manual/)
Mycobacterium Tuberculosis in a Hollow Fiber System
731