Fig. 3 Overall scheme for (a) infection of cells in suspension and (b) infection of plated cells
Table 1
Recommended conditions for siRNA transfection in 384-well microplate
Cell type
Transfection
reagent name
Transfection reagent
volume/well
DPBS
volume/
well
siRNA final
concentration
Cells/
well
Medium
RAW264.7 LTX
0.1 μL
9.9 μL
80 nM
2000
40 μL
a
BMDC
LTX
0.1 μL
9.9 μL
50 nM
40,000 40 μL
b
PBMC
CD14
+
Hiperfect
0.9 μL
9.1 μL
80 nM
20,000 40 μL
c
A549
RNAiMax
0.1 μL
9.9 μL
20 nM
1500
40 μL
a
THP-1
RNAiMax
0.1 μL
9.9 μL
50 nM
40,000 40 μL
a
a
Complete RPMI medium
b
Complete RPMI medium containing 40 ng/mL of murine GM-CSF
c
Complete RPMI medium. Supplement with 40 ng/mL of hM-CSF 6 h after cell plating
High-Content Analysis of Host-Pathogen Interactions
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