Editors
Tanya Parish
Center for Global Infectious Disease Research
Seattle Children’s Research Institute
Seattle, WA, USA
Anuradha Kumar
Center for Global Infectious Disease Research
Seattle Children’s Research Institute
Seattle, WA, USA
ISSN 1064-3745
ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-0716-1459-4
ISBN 978-1-0716-1460-0 (eBook)
https://doi.org/10.1007/978-1-0716-1460-0
© Springer Science+Business Media, LLC, part of Springer Nature 1998, 2009, 2015, 2021
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is
concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction
on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation,
computer software, or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply,
even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations
and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to
be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty,
expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been
made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Cover Illustration Caption: Trehalose probes specifically label the mycobacterial outer membrane, enabling a variety of
applications including live-cell imaging, proteomics experiments, and potentially diagnostic and therapeutic targeting.
The cover image depicts Mycobacterium smegmatis labeled with an azide-modified trehalose monomycolate probe and
then treated with an azide-reactive cyclooctyne fluorescent dye. Provided by the authors of Chapter 18.
This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer
Nature.
The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.
Tanya Parish
Center for Global Infectious Disease Research
Seattle Children’s Research Institute
Seattle, WA, USA
Anuradha Kumar
Center for Global Infectious Disease Research
Seattle Children’s Research Institute
Seattle, WA, USA
ISSN 1064-3745
ISSN 1940-6029 (electronic)
Methods in Molecular Biology
ISBN 978-1-0716-1459-4
ISBN 978-1-0716-1460-0 (eBook)
https://doi.org/10.1007/978-1-0716-1460-0
© Springer Science+Business Media, LLC, part of Springer Nature 1998, 2009, 2015, 2021
This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is
concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction
on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation,
computer software, or by similar or dissimilar methodology now known or hereafter developed.
The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply,
even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations
and therefore free for general use.
The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to
be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty,
expressed or implied, with respect to the material contained herein or for any errors or omissions that may have been
made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Cover Illustration Caption: Trehalose probes specifically label the mycobacterial outer membrane, enabling a variety of
applications including live-cell imaging, proteomics experiments, and potentially diagnostic and therapeutic targeting.
The cover image depicts Mycobacterium smegmatis labeled with an azide-modified trehalose monomycolate probe and
then treated with an azide-reactive cyclooctyne fluorescent dye. Provided by the authors of Chapter 18.
This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer
Nature.
The registered company address is: 1 New York Plaza, New York, NY 10004, U.S.A.