11. Sterile hemostats.
12. Sterile forceps.
13. Sterile scalpels #10.
14. Sterile small surgical shears.
15. 50 mL Oakridge tubes.
16. Sterile cups.
17. Sterile Petri plates.
18. Sterile 10 and 5 mL glass pipettes.
19. 15 mL Tenbroeck handheld tissue homogenizer (glass).
20. Sterile 5 and 10 mL syringe.
21. Sterile 27 G needle.
22. Gauze.
2.11 Radiorespirometry of Live
M. leprae
1. Whatman #42 ashless filter paper cut into 2 Â 4 cm strips.
2. Liquid scintillation concentrate (PPO-POPOP toluene concentrate) (see Note 10).
3. PPO (see Note 10).
4. Triton X-100.
5. NaOH-MeOH (8 g NaOH in 100 mL methanol) (see Note
11).
6. Radiorespirometry media: To 99 mL of DI water, add 0.47 g
7H9 base, 0.1 g casein hydrolysate, 0.5 g BSA, 4000 IU catalase, and 1.0 mL ampicillin (5 mg/mL). Filter sterilize using
0.2 μm filter unit. DO NOT autoclave. Add 0.5 μCi
14 C
palmitic acid. Store at 4
C (see Note 12).
7. Ampicillin (5 mg/mL).
8. Sterile filter units (0.2 μm).
9. Palmitic acid [114
C] (100 μCi/mL) in ethanol.
10. Sterile 6.0 mL capacity glass vials with caps.
11. 20 mL liquid scintillation vials (plastic).
2.12 Infecting
Armadillos
with M. leprae
1. Inoculum: 1 Â 10
9 M. leprae in a maximum vol of 1 mL,
derived from athymic nude mouse footpads.
2. Ketamine, Vetone zetamine (100 mg/mL) dosage, 0.6 mL
given intramuscularly (IM).
3. Dexmedetomidine: Dexdomitor (0.5 mg/mL) dosage, 0.4 mL
given IM.
4. 70% ethanol.
5. Reagents to test anti-PGL-1 serum antibodies [41].
28
Elizabeth Wallace et al.
12. Sterile forceps.
13. Sterile scalpels #10.
14. Sterile small surgical shears.
15. 50 mL Oakridge tubes.
16. Sterile cups.
17. Sterile Petri plates.
18. Sterile 10 and 5 mL glass pipettes.
19. 15 mL Tenbroeck handheld tissue homogenizer (glass).
20. Sterile 5 and 10 mL syringe.
21. Sterile 27 G needle.
22. Gauze.
2.11 Radiorespirometry of Live
M. leprae
1. Whatman #42 ashless filter paper cut into 2 Â 4 cm strips.
2. Liquid scintillation concentrate (PPO-POPOP toluene concentrate) (see Note 10).
3. PPO (see Note 10).
4. Triton X-100.
5. NaOH-MeOH (8 g NaOH in 100 mL methanol) (see Note
11).
6. Radiorespirometry media: To 99 mL of DI water, add 0.47 g
7H9 base, 0.1 g casein hydrolysate, 0.5 g BSA, 4000 IU catalase, and 1.0 mL ampicillin (5 mg/mL). Filter sterilize using
0.2 μm filter unit. DO NOT autoclave. Add 0.5 μCi
14 C
palmitic acid. Store at 4
C (see Note 12).
7. Ampicillin (5 mg/mL).
8. Sterile filter units (0.2 μm).
9. Palmitic acid [114
C] (100 μCi/mL) in ethanol.
10. Sterile 6.0 mL capacity glass vials with caps.
11. 20 mL liquid scintillation vials (plastic).
2.12 Infecting
Armadillos
with M. leprae
1. Inoculum: 1 Â 10
9 M. leprae in a maximum vol of 1 mL,
derived from athymic nude mouse footpads.
2. Ketamine, Vetone zetamine (100 mg/mL) dosage, 0.6 mL
given intramuscularly (IM).
3. Dexmedetomidine: Dexdomitor (0.5 mg/mL) dosage, 0.4 mL
given IM.
4. 70% ethanol.
5. Reagents to test anti-PGL-1 serum antibodies [41].
28
Elizabeth Wallace et al.