Table 1
PAM sequence table ordered by CRISPRi knockdown strength of a Renilla luciferase target in
M. smegmatis. sgRNAs targeting a modified Renilla luciferase 5
0 UTR were designed such that each
PAM sequence was tested against the same sgRNA targeting sequence. Following CRISPRi induction,
Renilla luciferase activity was measured and the fold repression in light production was determined.
Note that the closer the PAM sequence to the consensus 5
0 -NNAGAAW-3
0 [13], the stronger on
average was the observed target silencing. SD ¼ standard deviation. (Table reproduced from Rock
et al. 2017 [9])
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