Catalase
Using a sterile inoculating
loop, remove a small
amount of bacterial growth
from agar and smear on a
microscope slide
Performed when visible
growth is evident
Immediately before
performing the test,
prepare a 1:1 mixture of
10% Tween and 30%
H
2 O
2 . Add 1–2 drops of
the reagent to the test
culture
Bubbles
No bubbles
pH 7.0, 68
C
catalase
Emulsify several colonies in
0.5 mL of pH 7 phosphate
buffer
68
C for 20 min, then
remove from heat and cool
to room temperature
Immediately before
performing the test,
prepare a 1:1 mixture of
10% Tween and 30%
H
2 O
2 . Add 0.5 mL the
reagent to the test culture
Bubbles (within
20 min)
No bubbles
(within 20 min)
Culturing Mycobacteria
19