Table 1
Selectable markers and reported electroporation efficiencies for mycobacteria
Species
Selection
Efficiency
a
References
Fast growers
M. aurum
Kanamycin
100
[6, 25]
Streptomycin
[6, 25]
M. fortuitum
Kanamycin
[5]
M. parafortuitum
Kanamycin
300
[6]
Streptomycin
30
[6]
M. phlei
Kanamycin
[5]
M. smegmatis ATCC607
Kanamycin
10
[26]
M. smegmatis mc
2 155
Kanamycin
10
5 to 10
6
[19]
Hygromycin
5 Â 10
3
[4]
Apramycin
2 Â 10
4
[11]
Streptomycin
[6]
Gentamicin
[15]
M. vaccae
Hygromycin
10
3 to 10
5
[3, 4]
Slow-growers
M. avium
Kanamycin
10
2 to 10
4
[9, 10]
Hygromycin
10
4
[10]
M. avium ssp paratuberculosis
Kanamycin
100
[27]
M. bovis BCG
Kanamycin
10
3 to 10
5
[2]
Apramycin
10
3
[11]
Hygromycin
[4]
M. intracellulare
Kanamycin
[7, 28]
Gentamicin
[28]
M. marinum
Kanamycin
100
[8]
M. tuberculosis
Kanamycin
10
4 to 10
6
[2]
Apramycin
10
2
[11]
Hygromycin
10
7
[29]
M. w
Hygromycin
10
3 to 10
5
[4]
a
Number of transformants per μg of DNA
Electroporation
275