21. Paixa ˜o L, Rodrigues L, Couto I et al (2009)
Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli. J Biol
Eng 3:18
22. Rodrigues L, Ramos J, Couto I et al (2011)
Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance. BMC Microbiol 11:35
23. Rodrigues L, Villellas C, Bailo R et al (2013)
Role of the Mmr efflux pump in drug resistance
in Mycobacterium tuberculosis. Antimicrob
Agents Chemother 57:751–757
24. Machado D, Coelho TS, Perdiga ˜o J et al
(2017) Interplay between mutations and efflux
in drug resistant clinical isolates of Mycobacterium tuberculosis. Front Microbiol 8:711
25. Rodrigues L, Machado D, Couto I et al (2012)
Contribution of efflux activity to isoniazid
resistance in the Mycobacterium tuberculosis
complex. Infect Genet Evol 12:695–700
26. Ballister ER, Samanovic MI, Darwin KH
(2019) Mycobacterium tuberculosis Rv2700
contributes to cell envelope integrity and virulence. J Bacteriol 201:e00228–e00219
27. Xu W, DeJesus MA, Ru ¨cker N et al (2017)
Chemical genetic interaction profiling reveals
determinants of intrinsic antibiotic resistance
in Mycobacterium tuberculosis. Antimicrob
Agents Chemother 61:e01334–e01317
28. Williams EA, Mba Medie F, Bosserman RE
et al (2017) A nonsense mutation in Mycobacterium marinum that is suppressible by a novel
mechanism. Infect Immun 85:e00653–e00616
29. Aguilar-Pe ´rez C, Gracia B, Rodrigues L et al
(2018) Synergy between circular bacteriocin
AS-48 and ethambutol against Mycobacterium
tuberculosis. Antimicrob Agents Chemother
62:e00359–e00318
30. Mori G, Orena BS, Franch C et al (2018) The
EU approved antimalarial pyronaridine shows
antitubercular activity and synergy with
rifampicin, targeting RNA polymerase. Tuberculosis (Edinb) 112:98–109
31. Bonnett SA, Ollinger J, Chandrasekera S et al
(2016) A target-based whole cell screen
approach to identify potential inhibitors of
Mycobacterium tuberculosis signal peptidase.
ACS Infect Dis 2:893–902
32. Nakamura de Vasconcelos SS, Caleffi-Ferracioli
KR, Hegeto LA et al (2018) Carvacrol activity
& morphological changes in Mycobacterium
tuberculosis. Future Microbiol 13:877–888
33. Machado D, Pires D, Perdiga ˜o J et al (2016)
Ion channel blockers as antimicrobial agents,
efflux inhibitors, and enhancers of macrophage
killing activity against drug resistant Mycobacterium tuberculosis. PLoS One 11:e0149326
34. Palomino JC, Martin A, Camacho M et al
(2002) Resazurin microtiter assay plate: simple
and inexpensive method for detection of drug
resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 46:2720–2722
35. Costa SS, Lopes E, Azzali E et al (2016) An
experimental model for the rapid screening of
compounds with potential use against mycobacteria. Assay Drug Dev Technol 14:524–534
36. Pen ˜uelas-Urquides K, Villarreal-Trevin ˜o L,
Silva-Ramı ´rez B et al (2013) Measuring of
Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony
forming units. Braz J Microbiol 44:287–289
37. Rakhmawatie MD, Wibawa T, Lisdiyanti P et al
(2019) Evaluation of crystal violet decolorization assay and resazurin microplate assay for
antimycobacterial screening. Heliyon 5:
e02263
38. Franzblau SG, DeGroote MA, Cho SH et al
(2012) Comprehensive analysis of methods
used for the evaluation of compounds against
Mycobacterium
tuberculosis.
Tuberculosis
(Edinb) 92:453–488
Efflux and Permeability
245
Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli. J Biol
Eng 3:18
22. Rodrigues L, Ramos J, Couto I et al (2011)
Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlation with antibiotic resistance. BMC Microbiol 11:35
23. Rodrigues L, Villellas C, Bailo R et al (2013)
Role of the Mmr efflux pump in drug resistance
in Mycobacterium tuberculosis. Antimicrob
Agents Chemother 57:751–757
24. Machado D, Coelho TS, Perdiga ˜o J et al
(2017) Interplay between mutations and efflux
in drug resistant clinical isolates of Mycobacterium tuberculosis. Front Microbiol 8:711
25. Rodrigues L, Machado D, Couto I et al (2012)
Contribution of efflux activity to isoniazid
resistance in the Mycobacterium tuberculosis
complex. Infect Genet Evol 12:695–700
26. Ballister ER, Samanovic MI, Darwin KH
(2019) Mycobacterium tuberculosis Rv2700
contributes to cell envelope integrity and virulence. J Bacteriol 201:e00228–e00219
27. Xu W, DeJesus MA, Ru ¨cker N et al (2017)
Chemical genetic interaction profiling reveals
determinants of intrinsic antibiotic resistance
in Mycobacterium tuberculosis. Antimicrob
Agents Chemother 61:e01334–e01317
28. Williams EA, Mba Medie F, Bosserman RE
et al (2017) A nonsense mutation in Mycobacterium marinum that is suppressible by a novel
mechanism. Infect Immun 85:e00653–e00616
29. Aguilar-Pe ´rez C, Gracia B, Rodrigues L et al
(2018) Synergy between circular bacteriocin
AS-48 and ethambutol against Mycobacterium
tuberculosis. Antimicrob Agents Chemother
62:e00359–e00318
30. Mori G, Orena BS, Franch C et al (2018) The
EU approved antimalarial pyronaridine shows
antitubercular activity and synergy with
rifampicin, targeting RNA polymerase. Tuberculosis (Edinb) 112:98–109
31. Bonnett SA, Ollinger J, Chandrasekera S et al
(2016) A target-based whole cell screen
approach to identify potential inhibitors of
Mycobacterium tuberculosis signal peptidase.
ACS Infect Dis 2:893–902
32. Nakamura de Vasconcelos SS, Caleffi-Ferracioli
KR, Hegeto LA et al (2018) Carvacrol activity
& morphological changes in Mycobacterium
tuberculosis. Future Microbiol 13:877–888
33. Machado D, Pires D, Perdiga ˜o J et al (2016)
Ion channel blockers as antimicrobial agents,
efflux inhibitors, and enhancers of macrophage
killing activity against drug resistant Mycobacterium tuberculosis. PLoS One 11:e0149326
34. Palomino JC, Martin A, Camacho M et al
(2002) Resazurin microtiter assay plate: simple
and inexpensive method for detection of drug
resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 46:2720–2722
35. Costa SS, Lopes E, Azzali E et al (2016) An
experimental model for the rapid screening of
compounds with potential use against mycobacteria. Assay Drug Dev Technol 14:524–534
36. Pen ˜uelas-Urquides K, Villarreal-Trevin ˜o L,
Silva-Ramı ´rez B et al (2013) Measuring of
Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony
forming units. Braz J Microbiol 44:287–289
37. Rakhmawatie MD, Wibawa T, Lisdiyanti P et al
(2019) Evaluation of crystal violet decolorization assay and resazurin microplate assay for
antimycobacterial screening. Heliyon 5:
e02263
38. Franzblau SG, DeGroote MA, Cho SH et al
(2012) Comprehensive analysis of methods
used for the evaluation of compounds against
Mycobacterium
tuberculosis.
Tuberculosis
(Edinb) 92:453–488
Efflux and Permeability
245