Table 1
MALDI-TOF Mass Spectrometry of the individual types of mycolic acid methyl esters (MAME) of taxonomic interest, from representative mycobacterial
species
Species
MAME
Total carbon number of free acid
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
M. tuberculosis
α1146
1174
1202
1230
Methoxy1262
1290
1318
1346
Keto1246
1274 1288
1302 1316 1330
Hydroxya
1248
1276
1304
M. phlei
α1146
1160 1174 1188 1202 1216
Keto1162 1176
1190 1204
1218 1232 1246 1260
Waxb
1248 1262 1276 1290 1304
M. smegmatis
α1132 1146 1160
1174 1188
1202 1216 1230
Epoxy1148 1162
1176 1190 1204
1218 1232 1246 1260 1274
M. alvei
α1132 1146
1160 1174 1188 1202 1216
Methoxyc
1148
1162 1176
1190 1204
1218
Values represent the pseudo-molecular mass of mycolic acid methyl esters [M+Na].
+
The mass values of the major homologues are in bold.
a
Hydroxy-MA present in trace amount in H
37 Rv.
b
Wax-ester-MA at the origin of dicarboxylic MA (see
Note 10a).
c
ωÀ1 methoxy-MA
Lipid and Lipoarabinomannan
135