B
A
c
...a
...a
c
TDM
TMM
PG
Ac2PIM2
AcPIM2
Ac2PIM6
AcPIM6
C
D
E
PDIM A
PDIM B
TAG
S
P
GPL
TDM
Fig. 3 TLC of mycolic acid methyl esters (MAME) from some representative strains of mycobacteria. Roman
letters refer to mycolic acid types as in ref. [20, 52]: I α-; II α
0
; III methoxy-; IV keto-; V epoxy-; VI dicarboxylicmycolic acids. Lanes (a) M. fallax; (b) M. chelonae; (c) M. bovis BCG; (d) M. tuberculosis; (e) M. simiae; (f)
M. fortuitum; (g) M. xenopi; (h) M. avium; (i) M. vaccae. a means secondary alcohol, octadecanol and
eicosanol. Running solvents: (A): petroleum ether/diethyl ether 9:1 (v/v) four runs. (B) CH 2 Cl 2 . Revelation:
rhodamine B followed by charring. (C) TLC of “free” lipids of M. smegmatis after precipitation by methanol and
detection with anthrone reagent. S: soluble methanol, composed mainly of glycopeptidolipid (GPL). P: pellet,
enriched in trehalose dimycolate (TDM). Note the difference of coloration with anthrone between the intense
blue of GPL and gray-violet for TDM. Running solvent: CHCl 3 /CH 3 OH 9:1 (v/v). (D) TLC of “free” lipids of
M. tuberculosis H 37 Rv. Phthiocerol dimycocerosate (PDIM); triacyl glycerol (TAG). Running solvent: Petroleum
ether/ 9:1(v/v). Revelation: molybdophosphoric acid followed by charring. (E) TLC of “free” lipids of
M. tuberculosis H 37 Rv. Trehalose dimycolate (TDM); trehalose monomycolate (TMM); phosphatidyl glycerol
(PG); phosphatidyl inositol dimannoside diacylated (Ac 2 PIM 2 ); phosphatidyl inositol dimannoside monoacylated (AcPIM 2 ); phosphatidyl inositol hexamannoside diacylated (Ac 2 PIM 6 ); phosphatidyl inositol hexamannoside monoacylated (AcPIM 6 ). CHCl 3 :CH 3 OH:H 2 O 60:35:8 (v/v/v). Revelation: anthrone
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125
A
c
...a
...a
c
TDM
TMM
PG
Ac2PIM2
AcPIM2
Ac2PIM6
AcPIM6
C
D
E
PDIM A
PDIM B
TAG
S
P
GPL
TDM
Fig. 3 TLC of mycolic acid methyl esters (MAME) from some representative strains of mycobacteria. Roman
letters refer to mycolic acid types as in ref. [20, 52]: I α-; II α
0
; III methoxy-; IV keto-; V epoxy-; VI dicarboxylicmycolic acids. Lanes (a) M. fallax; (b) M. chelonae; (c) M. bovis BCG; (d) M. tuberculosis; (e) M. simiae; (f)
M. fortuitum; (g) M. xenopi; (h) M. avium; (i) M. vaccae. a means secondary alcohol, octadecanol and
eicosanol. Running solvents: (A): petroleum ether/diethyl ether 9:1 (v/v) four runs. (B) CH 2 Cl 2 . Revelation:
rhodamine B followed by charring. (C) TLC of “free” lipids of M. smegmatis after precipitation by methanol and
detection with anthrone reagent. S: soluble methanol, composed mainly of glycopeptidolipid (GPL). P: pellet,
enriched in trehalose dimycolate (TDM). Note the difference of coloration with anthrone between the intense
blue of GPL and gray-violet for TDM. Running solvent: CHCl 3 /CH 3 OH 9:1 (v/v). (D) TLC of “free” lipids of
M. tuberculosis H 37 Rv. Phthiocerol dimycocerosate (PDIM); triacyl glycerol (TAG). Running solvent: Petroleum
ether/ 9:1(v/v). Revelation: molybdophosphoric acid followed by charring. (E) TLC of “free” lipids of
M. tuberculosis H 37 Rv. Trehalose dimycolate (TDM); trehalose monomycolate (TMM); phosphatidyl glycerol
(PG); phosphatidyl inositol dimannoside diacylated (Ac 2 PIM 2 ); phosphatidyl inositol dimannoside monoacylated (AcPIM 2 ); phosphatidyl inositol hexamannoside diacylated (Ac 2 PIM 6 ); phosphatidyl inositol hexamannoside monoacylated (AcPIM 6 ). CHCl 3 :CH 3 OH:H 2 O 60:35:8 (v/v/v). Revelation: anthrone
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125