4. Florisil (60–100 mesh).
5. Glass test tubes.
6. Pasteur pipettes.
7. HPLC-grade solvents: Petroleum ether, ether, CHCl 3 , and
methanol (see Note 2).
8. Air or nitrogen stream.
2.12 Final
Purification of PDIM,
TDM, and GroMM by
Preparative TLC
1. Silica gel G60 precoated plates 20 Â 20 cm, 0.25 mm.
2. TLC tank with saturation paper.
3. 100-μL glass syringe.
4. Pasteur pipettes.
5. 1-cm Pasteur pipette equipped with cotton at the bottom and
filled with Florisil.
6. Glass test tubes.
7. HPLC-grade solvent: Petroleum ether, ether, and chloroform
(see Note 2).
8. Rhodamine B in ethanol diluted to 0.01% in 0.25 M NaH 2 PO 4
solution.
9. Air or nitrogen stream.
2.13 Purification of
Defined Nonpolar
Lipids by Puriflash
System (PDIM-A,
PDIM-B, and TAG)
1. Chromatoflash equipped of a 5-mL glass injection syringe.
2. Puriflash silica column 15-μm 4 g.
3. HPLC-grade solvents: Petroleum ether, diethylic ether, chloroform, and methanol (see Note 2).
4. Air or nitrogen stream.
5. 50 tubes of 22 mL (16 Â 150 mm) glass tubes (to collect
elutions).
6. Primulin 0.01% in water/acetone 1/4 v/v.
2.14 Final
Purification of MAME
by Preparative TLC
1. TLC tank with saturated paper.
2. Silica gel G60 precoated plate, 20 cm  20 cm 0.25 mm.
3. Pasteur pipettes.
4. 0.01% rhodamine B in ethanol diluted to 0.01% in 0.25 M
NaH 2 PO 4 solution.
5. HPLC-grade solvents: Petroleum ether, diethyl ether, and
dichloromethane (see Note 2).
6. Glass test tubes (16 Â 150 mm).
7. 100-μL glass syringe.
8. 1-cm Florisil Pasteur pipette equipped with cotton at the
bottom.
9. Air or nitrogen stream.
120
Marie-Antoinette Lane ´ elle et al.
5. Glass test tubes.
6. Pasteur pipettes.
7. HPLC-grade solvents: Petroleum ether, ether, CHCl 3 , and
methanol (see Note 2).
8. Air or nitrogen stream.
2.12 Final
Purification of PDIM,
TDM, and GroMM by
Preparative TLC
1. Silica gel G60 precoated plates 20 Â 20 cm, 0.25 mm.
2. TLC tank with saturation paper.
3. 100-μL glass syringe.
4. Pasteur pipettes.
5. 1-cm Pasteur pipette equipped with cotton at the bottom and
filled with Florisil.
6. Glass test tubes.
7. HPLC-grade solvent: Petroleum ether, ether, and chloroform
(see Note 2).
8. Rhodamine B in ethanol diluted to 0.01% in 0.25 M NaH 2 PO 4
solution.
9. Air or nitrogen stream.
2.13 Purification of
Defined Nonpolar
Lipids by Puriflash
System (PDIM-A,
PDIM-B, and TAG)
1. Chromatoflash equipped of a 5-mL glass injection syringe.
2. Puriflash silica column 15-μm 4 g.
3. HPLC-grade solvents: Petroleum ether, diethylic ether, chloroform, and methanol (see Note 2).
4. Air or nitrogen stream.
5. 50 tubes of 22 mL (16 Â 150 mm) glass tubes (to collect
elutions).
6. Primulin 0.01% in water/acetone 1/4 v/v.
2.14 Final
Purification of MAME
by Preparative TLC
1. TLC tank with saturated paper.
2. Silica gel G60 precoated plate, 20 cm  20 cm 0.25 mm.
3. Pasteur pipettes.
4. 0.01% rhodamine B in ethanol diluted to 0.01% in 0.25 M
NaH 2 PO 4 solution.
5. HPLC-grade solvents: Petroleum ether, diethyl ether, and
dichloromethane (see Note 2).
6. Glass test tubes (16 Â 150 mm).
7. 100-μL glass syringe.
8. 1-cm Florisil Pasteur pipette equipped with cotton at the
bottom.
9. Air or nitrogen stream.
120
Marie-Antoinette Lane ´ elle et al.