Figure 3.8
60
Bacterial Cellulose
(a)
(b)
Figure 3.8
The.aerosol.bioreactor.(left.hand:.back;.right.hand:.front)..(Hornung.et.al..2007).
Shezad.et.al..(2009).recently.described fed-batch.cultivation.for.the.generation.of.BNC.sheets.by.G. hansenii PJK.under.static.cultivation.conditions.using.
a.chemically.defined.medium..They.used.waste.from.beer.fermentation.broth.
to.decrease.the.material.costs..For.the.fed-batch.cultivation,.500.ml.of.a.culture.
medium.were.initially.inoculated.in.a.3.l.jar.fermenter.(Kobiotech.Co.,.Korea).
without.impeller..Fresh.medium.was.fed.in.periodically..The.cultivation.was.
carried.out.at.3°C.applying.an.aeration.rate.of.1.vvm..Compared.to.a.batch.
cultivation.after.30.days.of.cultivation,.the.authors.described.an.increase.of.
two.to.three.times.the.BNC.production.compared.to.fed-batch.cultivation.
Weuster-Botz.et.al..(2011).recently.presented.a.lab-scale.membrane.bioreactor.prototype.that.can.be.used.to.produce.a.BNC.pellicle.with.a.homogeneous.
layered.structure..They.used.a.hydrophilic.microfiltration.polyethersulfone.
membrane.to.separate.the.bacteria.from.the.cultivation.medium.and.to.provide.mechanical.support.for.the.formation.of.the.BNC.layer.
Combination of Static and Moving Cultivation
Other.concepts.investigated.during.the.last.couple.of.years.include.a.combination.of.static.and.moving.cultivation.principles.
Static Cultivation with Moving Harvesting
One.of.these.approaches.was.suggested.by.Saikiri.et.al..(1998).as.a.method.
for.direct.harvesting.of.BNC.filaments.during.the.cultivation.of.G. xylinus..
60
Bacterial Cellulose
(a)
(b)
Figure 3.8
The.aerosol.bioreactor.(left.hand:.back;.right.hand:.front)..(Hornung.et.al..2007).
Shezad.et.al..(2009).recently.described fed-batch.cultivation.for.the.generation.of.BNC.sheets.by.G. hansenii PJK.under.static.cultivation.conditions.using.
a.chemically.defined.medium..They.used.waste.from.beer.fermentation.broth.
to.decrease.the.material.costs..For.the.fed-batch.cultivation,.500.ml.of.a.culture.
medium.were.initially.inoculated.in.a.3.l.jar.fermenter.(Kobiotech.Co.,.Korea).
without.impeller..Fresh.medium.was.fed.in.periodically..The.cultivation.was.
carried.out.at.3°C.applying.an.aeration.rate.of.1.vvm..Compared.to.a.batch.
cultivation.after.30.days.of.cultivation,.the.authors.described.an.increase.of.
two.to.three.times.the.BNC.production.compared.to.fed-batch.cultivation.
Weuster-Botz.et.al..(2011).recently.presented.a.lab-scale.membrane.bioreactor.prototype.that.can.be.used.to.produce.a.BNC.pellicle.with.a.homogeneous.
layered.structure..They.used.a.hydrophilic.microfiltration.polyethersulfone.
membrane.to.separate.the.bacteria.from.the.cultivation.medium.and.to.provide.mechanical.support.for.the.formation.of.the.BNC.layer.
Combination of Static and Moving Cultivation
Other.concepts.investigated.during.the.last.couple.of.years.include.a.combination.of.static.and.moving.cultivation.principles.
Static Cultivation with Moving Harvesting
One.of.these.approaches.was.suggested.by.Saikiri.et.al..(1998).as.a.method.
for.direct.harvesting.of.BNC.filaments.during.the.cultivation.of.G. xylinus..