Figure 3.5
2
3
4
1
5
6
14
6
15
16
7
8
1. Nozzle for inoculation 11. Air inlet
9
10
2. Steam manometer
3. Air outflow
4. Nozzle for injection
of acid and alkali inlet
12. Sampling nozzle
13. Air filter
14. Control box
15. Acid storehouse
10 6
17
5. Thermometer
6. A transfer pump
7. DO electrode
16. Alkali storehouse
17. A constanttemperature
water bath
12 11
18
8. pH electrode
9. Water jacket
10. Water jacket
inflow/outflow
18. Steam boiler
19. Compressor
20. Air flow meter
13
19
20
56
Bacterial Cellulose
bubble-column.reactors..Within.this.modified.airlift.reactor,.elliptical.BNC.
pellets. are. formed.. Their. size. depended. strongly. on. the. amount. of. dissolved.oxygen.in.the.broth..One.additional.possibility.of.agitated.culture.for.
producing. food-use. gelatinous. BNC. is. applying. a. two-stage. fermentation.
process:.a.cell-growth.stage.in.an.airlift.fermenter.(agitated.culture).was.followed.by.the.BNC.formation.stage.in.production.incubators.(static.culture).
(Okiyama.1992).
Recently.Song.et.al..(2009).used.a.modified.airlift-type.bubble-column.bioreactor.to.transfer.an.agitated.cultivation.process.of.BNC.into.pilot.scale..For.
this.they.constructed.a.50.L.spherical-type.bubble-column.bioreactor.with.
low.shear.stress.and.high.oxygen.transfer.rates.(see.Figure 3.5)..Saccharified.
food.waste.was.used.as.culture.medium.due.to.its.low.cost.
BNC.produced.in.agitated.culture.exhibits.microstructural.changes.compared.to.material.produced.by.static.cultivation,.namely,.a.lower.degree.of.
polymerization. and. lower. crystallinity. index,. allowing. for. easier. chemical. modification.. Despite. a. reduction. in. the. ultimate. BNC. yield. and. the.
formation.of.the.bacterial.cells,.several.agitated.cultivation.methods.produce.high.space–time.yields.and.produce.various.shapes.and.structures..
Accordingly,.this.kind.of.cultivation.constitutes.an.important.basis.for.the.
diversity.of.the.high-tech.biopolymer.BNC.and.opens.up.novel.potential.
application.fields.
Figure 3.5
L.spherical-type.bubble-column.bioreactor.(Song.et.al..2009).
2
3
4
1
5
6
14
6
15
16
7
8
1. Nozzle for inoculation 11. Air inlet
9
10
2. Steam manometer
3. Air outflow
4. Nozzle for injection
of acid and alkali inlet
12. Sampling nozzle
13. Air filter
14. Control box
15. Acid storehouse
10 6
17
5. Thermometer
6. A transfer pump
7. DO electrode
16. Alkali storehouse
17. A constanttemperature
water bath
12 11
18
8. pH electrode
9. Water jacket
10. Water jacket
inflow/outflow
18. Steam boiler
19. Compressor
20. Air flow meter
13
19
20
56
Bacterial Cellulose
bubble-column.reactors..Within.this.modified.airlift.reactor,.elliptical.BNC.
pellets. are. formed.. Their. size. depended. strongly. on. the. amount. of. dissolved.oxygen.in.the.broth..One.additional.possibility.of.agitated.culture.for.
producing. food-use. gelatinous. BNC. is. applying. a. two-stage. fermentation.
process:.a.cell-growth.stage.in.an.airlift.fermenter.(agitated.culture).was.followed.by.the.BNC.formation.stage.in.production.incubators.(static.culture).
(Okiyama.1992).
Recently.Song.et.al..(2009).used.a.modified.airlift-type.bubble-column.bioreactor.to.transfer.an.agitated.cultivation.process.of.BNC.into.pilot.scale..For.
this.they.constructed.a.50.L.spherical-type.bubble-column.bioreactor.with.
low.shear.stress.and.high.oxygen.transfer.rates.(see.Figure 3.5)..Saccharified.
food.waste.was.used.as.culture.medium.due.to.its.low.cost.
BNC.produced.in.agitated.culture.exhibits.microstructural.changes.compared.to.material.produced.by.static.cultivation,.namely,.a.lower.degree.of.
polymerization. and. lower. crystallinity. index,. allowing. for. easier. chemical. modification.. Despite. a. reduction. in. the. ultimate. BNC. yield. and. the.
formation.of.the.bacterial.cells,.several.agitated.cultivation.methods.produce.high.space–time.yields.and.produce.various.shapes.and.structures..
Accordingly,.this.kind.of.cultivation.constitutes.an.important.basis.for.the.
diversity.of.the.high-tech.biopolymer.BNC.and.opens.up.novel.potential.
application.fields.
Figure 3.5
L.spherical-type.bubble-column.bioreactor.(Song.et.al..2009).