Figure 3.4
Gas outlet
Nozzle for inoculation
Nozzle for addition
of acid and base
Sight glass
Inlet and outlet
for temperature
Sensor nozzles
control water
Sensor nozzles
Sensor nozzles
Drain
Sampling nozzles
Inlet and outlet
for temperature
control water
Draft tube
Temperature sensor
Drain
54
Bacterial Cellulose
Within. this. bioreactor,. 12. PCS. tubes. were. bound. to. the. agitator. shaft. in. a.
gridlike.fashion,.with.six.rows.of.two.parallel.tubes..Batch.fermentation.in.
this. reactor. for. 120. h. using. the. best. suited. type. of. PCS. tubes. resulted. in.
higher.yields.and.improved.material.properties.of.the.BNC.generated.compared.to.suspended-cell.fermentation.used.as.control..For.example,.the.BNC.
tested.from.the.PCS.biofilm.reactor.showed.an.increase.in.the.stress.at.break.
from.2.8.to.34.2.MPa.(PCS-grown.BNC).and.of.the.Young’s.modulus.from.
286.to.2401.MPa.(PCS-grown.BNC).
Airlift reactors
Instead. of. a. stirred. bioreactor,. Chao. et. al.. (2000). successfully. used. a. 50. L.
internal-loop. airlift. bioreactor. (Figure 3.4). to. improve. the. oxygen. transfer.
rate.and.achieved.a.high.BNC.yield.of.3.8.g/l.after.67.h.(corresponding.productivity. 0.0567. g/l/h). using. G. xylinus subsp.. sucrofermentans (BPR2001)..
However,. the. production. rate. of. BNC. was. significantly. increased. by. the.
utilization.of.an.oxygen-enriched.gas.in.the.system..The.BNC.amount.rose.
to.0.116.g/l/h..Chao.et.al..pointed.out.that.the.value.is.twice.that.of.a.typical.air-supplied.culture.and.comparable.to.that.of.a.mechanically.agitated.
Figure 3.4
Schematic.representation.of.the.50.L.internal-loop.airlift.reactor.(Chao.et.al..2000).
Gas outlet
Nozzle for inoculation
Nozzle for addition
of acid and base
Sight glass
Inlet and outlet
for temperature
Sensor nozzles
control water
Sensor nozzles
Sensor nozzles
Drain
Sampling nozzles
Inlet and outlet
for temperature
control water
Draft tube
Temperature sensor
Drain
54
Bacterial Cellulose
Within. this. bioreactor,. 12. PCS. tubes. were. bound. to. the. agitator. shaft. in. a.
gridlike.fashion,.with.six.rows.of.two.parallel.tubes..Batch.fermentation.in.
this. reactor. for. 120. h. using. the. best. suited. type. of. PCS. tubes. resulted. in.
higher.yields.and.improved.material.properties.of.the.BNC.generated.compared.to.suspended-cell.fermentation.used.as.control..For.example,.the.BNC.
tested.from.the.PCS.biofilm.reactor.showed.an.increase.in.the.stress.at.break.
from.2.8.to.34.2.MPa.(PCS-grown.BNC).and.of.the.Young’s.modulus.from.
286.to.2401.MPa.(PCS-grown.BNC).
Airlift reactors
Instead. of. a. stirred. bioreactor,. Chao. et. al.. (2000). successfully. used. a. 50. L.
internal-loop. airlift. bioreactor. (Figure 3.4). to. improve. the. oxygen. transfer.
rate.and.achieved.a.high.BNC.yield.of.3.8.g/l.after.67.h.(corresponding.productivity. 0.0567. g/l/h). using. G. xylinus subsp.. sucrofermentans (BPR2001)..
However,. the. production. rate. of. BNC. was. significantly. increased. by. the.
utilization.of.an.oxygen-enriched.gas.in.the.system..The.BNC.amount.rose.
to.0.116.g/l/h..Chao.et.al..pointed.out.that.the.value.is.twice.that.of.a.typical.air-supplied.culture.and.comparable.to.that.of.a.mechanically.agitated.
Figure 3.4
Schematic.representation.of.the.50.L.internal-loop.airlift.reactor.(Chao.et.al..2000).