Figure 2.8
 
 
TABLe 2.1
The.Cellulose.Production.Yield.is.Presented.
in.Both.Grams.of.Bacterial.Cellulose.
Obtained.per.Consumed.Grams.of.Fructose.
and.as.Yield.Obtained.Per.Day;.pH.5.5.
Yield p/s
Production Rate p/s
(g/g)
(g/g/day)
Day.1
2.402
2.402
Day.2
0.541
0.271
Day.3
0.183
0.061
Day.4
0.145
0.036
Day.7
0.141
0.02
35
Effect of Cultivation on BNC Biomaterials
(a)
50 µm
(b)
50 µm
(c)
50 µm
Figure 2.8
(See color insert.).Microscopy.images.showing.radial.distributions.of.cellulose.fibers.(blue,.
a and.c).and.G. xylinus. (red,.b).measured.on.2-day-old.tubes.by.SHG.and.CARS.microscopy,.
respectively..Note.the.variations.in.cellulose.density.and.SHG.image.intensity.between.regions.
on.the.inner.luminal.side.(left).and.outer.side.(right).observed.for.both.a.tube.taken.directly.
from.. cultivation.(a).and.a.tube.after.purification.(c).
. density..A  layered.cellulose.arrangement,.with.a.dense.region.of.high.SHG.signal.at.the.luminal.(left).side,.can.also.be.seen.in.the.SHG.image.of.Figure  2.8C,.
. measured.on.a.2-day-old.cellulose.tube.after.material.purification.
Cellulose Production Yield and Cellulose Content
The. fructose. level. in. the. cultivation. medium,. before. and. after. cultivation,.
and.the.cellulose.dry.weight.were.used.to.calculate.the.yield.and.production.
rate.of.bacterial.cellulose.(Table 2.1)..The.yield.decreased.during.the.7.days.of.
cultivation.and.the.highest.yield.was.obtained.at.day.1.(2.402.g/g)..This.high.
yield.is.probably.a.result.of.the.yield.being.calculated.only.for.the.fructose.
utilization. and. not. the. small. amount. of. glucose. present. in. the. cultivation.
medium.in.the.first.48.hours.(Table 2.1).