TABLe 11.2
Assignment.of.ATR-FTIR.Spectra.for.BC.Templates.Soaking.in.1.5.SBF.Solution.for.
21.Days
Wavenumber (cm -1 )
Assignment
0 Days
2 Days
7 Days
14 Days
21 Days
OH,.υ.(BC)
HPO 4
2− .(OCP)
PO 4
3− ,.υ 3(asym)
PO 4
3− ,.υ 1(sym)
HPO 4
2− .(OCP)
CO 3
2−
PO 4
3− ,.υ 4a
PO 4
3− ,.υ 4c
~3448
~3448
1295–1297
1020–1024
910–911
~3448
1295–1297
1020–1024
910–911
873–875
600–602
558–560
~3448 a
1020–1024 b
960–962 .b
873–875
600–602 .b
558–560 .b
~3448 a
1020–1024 b
960–962 .b
873–875
600–602 .b
558–560 .b
a. The.OH.absorption.band.of.BC.became.very.weak.with.increasing.soaking.time.
b. The.PO 4
3− .absorption.band.of.apatite.became.strong.with.increasing.soaking.time.
Source:. Data. from. T.. T.. Nge. and. J.. Sugiyama,. “Surface. functional. groups. dependent. apatite.
formation. on. bacterial. cellulose. microfibrils. network. in. a. simulated. body. fluid,”.
J. Biomed. Mater. Res..81A.(2007):.124–134.
230
Bacterial Cellulose
phate.(ACP).(Termine.and.Posner.1966),.brushite.(dicalcium.phosphate.dihydrate.[DCPD]).(Francis.and.Webb.1971),.and.octacalcium.phosphate.(OCP).
(Houllé.et.al..1998).because.they.are.kinetically.favorable.to.deposit.under.
the.same.conditions.with.respect.to.apatite..One.or.a.combination.of.these.
precursor.phases.eventually.transform.into.a.mature.Ca-P.phase.of.biological.hydroxyapatite,.which.is.regarded.as.the.most.thermodynamically.stable.
Ca-P.phase.in.the.physiological.environment.
The.detailed.information.relative.to.the.Ca-P.phases.obtained.with.soaking.times.of.2,.7,.14,.and.21.days.was.revealed.by.ATR-FTIR.spectral.features.
(Nge.and.Sugiyama.2007).and.the.characteristics.spectral.data.are.described.
in.Table 11.2..The.characteristic.phosphate.absorption.bands.of.the.Ca-P.phase.
(Barrère.et.al..2003;.Klee.and.Engel.1970;.Koutsopoulos.2002).at.1020–1024/cm.
3−
3−
3−
(PO 4 ,.υ 3 ),.600–602/cm.(PO 4 ,.υ 4a ),.and.558-560/cm.(PO 4 ,.υ 4c ).were.eventually.detected.with.increasing.soaking.time..The.phosphate.absorption.band.
at.1020–1024/cm.is.attributed.to.the.presence.of.crystalline.imperfection.in.
the. apatite. structure,. that. is,. nonstoichiometric. apatite. containing. HPO 4
2− .
and/or. CO 3
2− . groups. (Rey. et. al.. 1991b),. whereas. the. characteristic. phosphate.absorption.band.of.stoichiometric.hydroxyapatite.usually.displays.at.
approximately.1030/cm..Moreover,.the.characteristic.HPO 4
2− .bands.of.OCP.
at.1295–1297/cm.and.910–911/cm.corresponding.to.OH.in-plane.bending.and.
P-OH.stretching.mode.of.HPO 4 (6).(Barrère.et.al..2003;.Fowler.et.al..1993).were.
detected.at.2-day.and.7-day.spectra.and.became.weak.and/or.disappeared.
at.the.14-day.and.21-day.spectra..It.was.reported.(Fowler.et.al..1993).that.the.
HPO 4 (6).in.OCP.preferentially.decreased.during.the.progressive.hydrolysis.