2000
2000(5)
AJ2000
2000B
Acetobacter aceti
(AJ 12368)
0416
1010
1604
1604(5)
1010(5)
0416(5)
M0416A
M1010A
M1604A
0416B
1010B
1604B
0020
0020(5)
M0020A
0020B
Figure 11.1
TABLe 11.1
Composition.of.Glucose.and.N-Acetylglucosamine.(GlcNAc).at.a.Fixed.
Carbon.Source.of.2%
Medium ID a
Glucose (g/100 ml)
GlcNAc (g/100 ml)
SH.medium
2000
2.0
0.0
Modified.SH.medium
1604
1.6
0.4
Modified.SH.medium
1010
1.0
1.0
Modified.SH.medium
0416
0.4
1.6
Modified.SH.medium
0020
0.0
2.0
a. A.medium.ID.was.given.for.the.different.sugar.compositions..The.BC.produced.
from.the.different.media.are.named.after.the.corresponding.medium.ID.
Source:. Adapted. from. T.. T.. Nge. et. al.,. Bacterial. cellulose-based. biomimetic.
composites. ..In:.Biopolymers.(ISBN.978-953-307-109-1,.InTech,.2010),.345–368,.
with.permission.
222
Bacterial Cellulose
Figure 11.1
Experimental.setup.for.N-acetylglucosamine.(GlcNAc).incorporation.in.BC..The.first.two.digits. .
represent.the.glucose.concentration.and.the.later.two.represent.the.GlcNAc.concentration.as.
described. in. Table. 1.. M:. modified. medium. containing. GlcNAc. (starter. culture. obtained. by.
5 times.repeated.transferring.of.inoculum.at.3.days.of.growth)..A:.GlcNAc-containing.medium/
GlcNAc-containing.BC.(inoculation.using.the.same.sugar.contents.as.for.the.starter.culture)..
B:. GlcNAc-containing. BC. with. different. sugar. content. (inoculation. from. AJ2000,. M1604-A,.
and.M0020-A.as.starter.cultures)..Source: From.Nge.et.al.,.Biopolymers.(ISBN:978-953-307-109-1,.
InTech.,.2010),.345–368,.with.permission.
until.day.14..In.the.presence.of.mixed.sugars.(glucose.and.GlcNAc),.the.lag.
phase.was.shorter.than.that.of.AJ2000.medium.as.judged.by.a.higher.mass.
production.at.days.3.and.4,.followed.by.an.increasing.mass.phase.until.day 7.
ars.media.was.lower.than.AJ2000.medium,.with.4.74,.3.33,.and.1.29.mg.cellulose/ml.for.M1604-1604A,.M1604-1010B,.and.M1604-0416B,.respectively..The.
cellulose.production.in.the.medium.containing.GlcNAc.only.(M1604-0020B).
was.low.(0.19.mg/ml).throughout.the.incubation.period,.most.likely.reflecting. low. cell. growth.. At. a. fixed. total. carbon. source. of. 2%,. the. decrease. in.
cellulose. mass. with. increasing. GlcNAc. concentrations. reflects. the. limitation.of.cellulose.formation,.although.GlcNAc.enhances.the.initial.cellulose.
production. rate.. There. was. greater. consumption. of. glucose. rather. than.
GlcNAc.by.the.cells..The.mass.therefore.decreased.with.decreasing.glucose.