11
Biomimetic Mineralization of
Apatite on Bacterial Cellulose
Thi Thi Nge 1,2 and Junji Sugiyama 2
1 Institute of Scientific and Industrial Research (ISIR),
Osaka University, Ibaraki, Osaka, Japan
2 Research Institute for Sustainable Humanosphere (RISH),
Kyoto University, Gokasho, Uji, Kyoto, Japan
CONTENTS
Introduction.......................................................................................................... 218
Preparation.of.BC.Templates. ............................................................................. 220
Microbial.Synthesis.of.BC.from.Standard.Culture.Medium.
Microbial.Synthesis.of.BC.from.Modified.Culture.Medium.
(Native BC). ...................................................................................................... 220
Surface.Modification.by.TEMPO-Mediated.Oxidation.(BC-TEMPO). .... 221
(BC-GlcNAc).................................................................................................... 221
Morphology.of.BC.Templates. .......................................................................225
Biomimetic.Mineralization.of.Apatite.on.BC.Templates. ............................... 227
Soaking.BC.Templates.in.an.SBF.(1.5.SBF).................................................. 227
Soaking.BC.Templates.in.Calcium.and.Phosphate.Solutions................... 227
Morphology.and.Structural.Analyses of the.Mineralized.Ca-P.Phase........ 228
BC.Templates.Soaked.in.1.5.SBF................................................................... 228
BC.Templates.Alternatively.Soaked.in.Calcium.and.Phosphate.
Solutions........................................................................................................... 231
Conclusion............................................................................................................234
Acknowledgments............................................................................................... 235
References. ............................................................................................................. 236
Biomimetic. mineralization. of. apatite. on. bacterial. cellulose. (BC). was.
investigated. by. soaking. different. modified. BC. templates. in. a. simulated. body. fluid. (1.5. SBF). and/or. alternate. soaking. in. 0.05. M. calcium.
chloride.and.0.03.M.sodium.dihydrogen.phosphate.solutions.prepared.
with. a. calcium:phosphorus. molar. ratio. of. 1.67. at. a. physiological. temperature. of. 37°C.. Modification. of. BC. includes. surface. modification. by.
217
Biomimetic Mineralization of
Apatite on Bacterial Cellulose
Thi Thi Nge 1,2 and Junji Sugiyama 2
1 Institute of Scientific and Industrial Research (ISIR),
Osaka University, Ibaraki, Osaka, Japan
2 Research Institute for Sustainable Humanosphere (RISH),
Kyoto University, Gokasho, Uji, Kyoto, Japan
CONTENTS
Introduction.......................................................................................................... 218
Preparation.of.BC.Templates. ............................................................................. 220
Microbial.Synthesis.of.BC.from.Standard.Culture.Medium.
Microbial.Synthesis.of.BC.from.Modified.Culture.Medium.
(Native BC). ...................................................................................................... 220
Surface.Modification.by.TEMPO-Mediated.Oxidation.(BC-TEMPO). .... 221
(BC-GlcNAc).................................................................................................... 221
Morphology.of.BC.Templates. .......................................................................225
Biomimetic.Mineralization.of.Apatite.on.BC.Templates. ............................... 227
Soaking.BC.Templates.in.an.SBF.(1.5.SBF).................................................. 227
Soaking.BC.Templates.in.Calcium.and.Phosphate.Solutions................... 227
Morphology.and.Structural.Analyses of the.Mineralized.Ca-P.Phase........ 228
BC.Templates.Soaked.in.1.5.SBF................................................................... 228
BC.Templates.Alternatively.Soaked.in.Calcium.and.Phosphate.
Solutions........................................................................................................... 231
Conclusion............................................................................................................234
Acknowledgments............................................................................................... 235
References. ............................................................................................................. 236
Biomimetic. mineralization. of. apatite. on. bacterial. cellulose. (BC). was.
investigated. by. soaking. different. modified. BC. templates. in. a. simulated. body. fluid. (1.5. SBF). and/or. alternate. soaking. in. 0.05. M. calcium.
chloride.and.0.03.M.sodium.dihydrogen.phosphate.solutions.prepared.
with. a. calcium:phosphorus. molar. ratio. of. 1.67. at. a. physiological. temperature. of. 37°C.. Modification. of. BC. includes. surface. modification. by.
217