TABLE 7.9
Core Components of the Notch Pathway in Xenopus Neural Border and Its Descendants
krt12.4, non-neural ectoderm marker; nkx1–2, posterior NB marker ( Kurata and Ueno, 2003 ); sox2, sox3, soxd, neural plate markers; foxe3, positive regulator of lens fate ( Ogino et al., 2008 ); pax3, msx1, zic1,
NB markers ( Nichane et al., 2008b ). For additional abbreviations, see main text and Table 7.3 legend.
Expression
Gain-of-Function
Loss-of-Function
(Dominant-Negative/Morpholino)
jag1
dlc
dll1
notch2
notch1/RBPJ
Gene
notch1 expression: NB, neural plate, and ectoderm (Coffman
et al., 1993). Prospective NCC territories (neural plate
stage) (Glavic et al., 2004).
NB, NCC:
notch1ΔE : ↓twist1, nkx1–2, non-neural ectoderm (krt12.4)
(neural plate stage, ISH) (Coffman et al., 1993; Kurata and
Ueno, 2003); ↓branchial arches (tadpole) (Coffman et al.,
1993 ).
NICD1 : ↑NB marker hes4 (gastrula, ISH) (López et al.,
2005 ), neural plate marker sox2 (neural plate stage, ISH)
(Revinski et al., 2010).
GR-NICD1 or GR-RBPJ-Ank (Dex NF12): ↑presumptive NCC
(snai2, foxd3, hes4); ↓bmp4 (non-neural ectoderm) (late
neurula) (Glavic et al., 2004).
GR-RBPJ-Ank (Dex NF11) : hes4NCC domain unaffected
(neural plate stage, ISH) ( Nichane et al., 2008a ).
PPE:
NICD1: ↑hes5.4 in placodes and adjacent non-neural
ectoderm, even in the absence of six1/eya1 function (ISH,
neural plate stage) (Riddiford and Schlosser, 2017).
Lens placode (notch1 /notch2 ?):
GR-RBPJ-VP16 (Dex NF15): ectopic foxe3 restricted to the
anterior ectoderm (NF22–24) (Ogino et al., 2008).
GR-RBPJ-VP16 or NICD1 + GR-otx2 (Dex NF15): massive
ectopic foxe3 (NF22–24) (Ogino et al., 2008).
NB, NCC:
RBPJ DBM :
↓neural plate (sox2) (neural plate stage, ISH)
(Revinski et al., 2010).
notch1 MO : ↓presumptive neural plate (sox2) (late gastrula);
↑
plate (
neural
sox2) (neural plate stage) (ISH) (Revinski et
al., 2010).
GR-RBPJ DBM (Dex NF12 ): ↓presumptive NCC (snai2,
foxd3, hes4); ↑bmp4 domain (late neurula) (Glavic et al.,
2004 ).
GR-RBPJ DBM (Dex NF11 ): hes4NCC domain unaffected
(neural plate stage, ISH) (Nichane
et al., 2008a)
PPE:
RBPJ DBM: ↓hes5.4 in neural plate and placodes (ISH, neural
plate stage) (Riddiford and Schlosser, 2017).
Lens placode (notch1 /notch2 ?):
GR-RBPJ DBM /Dex NF15: ↓foxe3, impaired lens development
(NF22–24) ( Ogino et al., 2008 ).
PPE (neural plate stage) ( Murato and Hashimoto, 2009 ;
Maharana and Schlosser, 2018 ).
Anterior neural border (ANB), surrounding the presumptive
NCC at early neurula stage. ( Glavic et al., 2004 ).
NB, NCC:
dll1 overexpression : ↑NCC (snai2, sox10) without affecting
NB (pax3, msx1), neural plate (soxd, sox3), or placodal
marker six1 ( Nichane et al., 2008b ).
NB, NCC:
dll1 STU: ↓NCC (snai2, foxd3, hes4); ↑bmp4 (late neurula)
( Glavic et al., 2004 ); ↓neural plate (sox2) (late gastrula,
early neurula; ISH) ( Revinski et al., 2010 ).
.
dll1 MO : ↓NCC (snai2, sox10) (neural plate stage) ( Nichane et
al., 2008b ).
Lens placode:
dll1 STU: Head defects, ↓foxe3 ( Ogino et al., 2008 )
Similar to dll1 but at lower levels. Expressed in an arc
NCC, placodes:
corresponding to the ANB; later resolves into dlc+ cranial
dlc MO: migration failure of NCC and placodal cells ( Peres
placodes ( Glavic et al., 2004 ; Peres and Durston, 2006 ).
and Durston, 2006 ).
dlc tr: ↓foxe3 (NF23) ( Ogino et al., 2008 ).
ANB, surrounding the presumptive NCC at early neurula
stage ( Glavic et al., 2004 ).
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Xenopus