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JOHN E. CUSHJNG
( also called “immunodiffusion” ) of demonstrating antigen-antibody reactions are in their variety of forms among the most useful, yet simple,
of those in current use. Their value is further enhanced by the coupling
of simple diffusion with electrophoresis to produce immunoelectrophoretic systems capable of resolutions of extreme specificity.
As in all methods, initial difficulties are invariably encountered in the
development of specific applications, but generally speaking, the careful
and persistent worker with general laboratory know-how will find that
with a reasonable expenditure of efforts, he will be rewarded.
The sections to follow are written with the assumption that the
reader initially will be concerned with observations and concepts and
will follow through the literature those citations of methods that are
of specific interest to him.
C. General References
Those who would like to read broadly on immunology will find a
number of books that have appeared recently. The following are suggested among the several that this writer considers useful: Kabat’s
“Structural Concepts in Immunology and Immunochemistry” ( 1968),
Haurowitz’s “Immunochemistry and the Biosynthesis of Antibodies”
( 196S), Hildemann’s “Immunogenetics” ( 1970), Pressman and Grossberg’s “The Structural Basis of Antibody Specificity” ( 196S), the annual
series” Advances in Immunology (1961 to date), the Cold Spring Harbor
Symposium, Volume XXXII, “Antibodies” ( 1W8), and the Oak Ridge
National Laboratory Symposium ( 1966).
11. ANTIBODIES
As noted in the Introduction, the study of the nature and synthesis
of antibodies, such as are found in the serums of humans and readily
available laboratory mammals, currently occupies the attention of so
many persons as to have bccome one of the major areas of interest
in biology. The principal reasons for this follow. First, the study of
antibody molecules forms the heart not only of immunochemistry but
of immunology in general. Second, the synthesis of antibodies cannot
yet be experimentally related to the so-called dogma of the genetic code
and therefore presents an outstanding challenge to those who would
understand the mechanism of protein synthesis in general. Third, the
differentiation during morphogenesis of the specialized antibody syn-
JOHN E. CUSHJNG
( also called “immunodiffusion” ) of demonstrating antigen-antibody reactions are in their variety of forms among the most useful, yet simple,
of those in current use. Their value is further enhanced by the coupling
of simple diffusion with electrophoresis to produce immunoelectrophoretic systems capable of resolutions of extreme specificity.
As in all methods, initial difficulties are invariably encountered in the
development of specific applications, but generally speaking, the careful
and persistent worker with general laboratory know-how will find that
with a reasonable expenditure of efforts, he will be rewarded.
The sections to follow are written with the assumption that the
reader initially will be concerned with observations and concepts and
will follow through the literature those citations of methods that are
of specific interest to him.
C. General References
Those who would like to read broadly on immunology will find a
number of books that have appeared recently. The following are suggested among the several that this writer considers useful: Kabat’s
“Structural Concepts in Immunology and Immunochemistry” ( 1968),
Haurowitz’s “Immunochemistry and the Biosynthesis of Antibodies”
( 196S), Hildemann’s “Immunogenetics” ( 1970), Pressman and Grossberg’s “The Structural Basis of Antibody Specificity” ( 196S), the annual
series” Advances in Immunology (1961 to date), the Cold Spring Harbor
Symposium, Volume XXXII, “Antibodies” ( 1W8), and the Oak Ridge
National Laboratory Symposium ( 1966).
11. ANTIBODIES
As noted in the Introduction, the study of the nature and synthesis
of antibodies, such as are found in the serums of humans and readily
available laboratory mammals, currently occupies the attention of so
many persons as to have bccome one of the major areas of interest
in biology. The principal reasons for this follow. First, the study of
antibody molecules forms the heart not only of immunochemistry but
of immunology in general. Second, the synthesis of antibodies cannot
yet be experimentally related to the so-called dogma of the genetic code
and therefore presents an outstanding challenge to those who would
understand the mechanism of protein synthesis in general. Third, the
differentiation during morphogenesis of the specialized antibody syn-