330
FINDLAY E. RUSSELL
shown in Fig. 11, and obtained a solution which on injection into
rabbits produced increased salivation, respiratory distress, clonic
convulsions, paralysis and death. The solution also hemolized rabbit
red blood cells.
50 ml eel serum
(49 mg protein/ml)
AmSO, 25% sat
/ 'I
Precipiiate
Supeinatant
AmSO, t o 35% sat
\
\
\
Precipitate
Supernatant
Redissolve in 50 ml NaCl 0.15 M
p H 5.5 = 2 mg proteinlml
25% ethanol at - 5°C
Precipitate
Sipernatant
Redissolve in 50 ml N a C l 0.15 M
and dialyze = 0.48 mg protein/ml
FIQ. 11. Fraotionation method for eel serum used by Ghiretti and Rocca (1963)
More recently, Rocca and Ghiretti (1964) fractionated the serum
proteins on a DEAE cellulose column and demonstrated that the lethal
activity was associated with a single fraction. Several fractions were
obtained by linear gradient elution from 0.02M at pH 7.5 to 0.2M
at pH 6.5 Tris-phosphate buffer. The fractions collected between pH
7-3 and 7.0 were found to contain 30 to 40% of the total proteins, and
the entire lethal activity.,
VIII. VENOMOUS FISHES
Fifteen years ago Dr. Halstead and I sat down one Monday afternoon in the old " Fish Museum " of the School of Tropical Medicine at
Lorna Linda and examined some notes I had prepared for a seminar at
FINDLAY E. RUSSELL
shown in Fig. 11, and obtained a solution which on injection into
rabbits produced increased salivation, respiratory distress, clonic
convulsions, paralysis and death. The solution also hemolized rabbit
red blood cells.
50 ml eel serum
(49 mg protein/ml)
AmSO, 25% sat
/ 'I
Precipiiate
Supeinatant
AmSO, t o 35% sat
\
\
\
Precipitate
Supernatant
Redissolve in 50 ml NaCl 0.15 M
p H 5.5 = 2 mg proteinlml
25% ethanol at - 5°C
Precipitate
Sipernatant
Redissolve in 50 ml N a C l 0.15 M
and dialyze = 0.48 mg protein/ml
FIQ. 11. Fraotionation method for eel serum used by Ghiretti and Rocca (1963)
More recently, Rocca and Ghiretti (1964) fractionated the serum
proteins on a DEAE cellulose column and demonstrated that the lethal
activity was associated with a single fraction. Several fractions were
obtained by linear gradient elution from 0.02M at pH 7.5 to 0.2M
at pH 6.5 Tris-phosphate buffer. The fractions collected between pH
7-3 and 7.0 were found to contain 30 to 40% of the total proteins, and
the entire lethal activity.,
VIII. VENOMOUS FISHES
Fifteen years ago Dr. Halstead and I sat down one Monday afternoon in the old " Fish Museum " of the School of Tropical Medicine at
Lorna Linda and examined some notes I had prepared for a seminar at