EXT RACTlO N
4 kg DRY FISH POWDER
EXTRACT WITH
ETHANOL. 48 hr
I REMOVE ETHANOL
2 DILUTE WITH WATER TO 4 liter
3 EXTRACT WITH DIETHYL ETHER
4 REMOVE ETHER
TREAT WITH ACETONE
ET ANOIC EXTRACT
1
ETHERAL EXTRACT RESIDUE
8.3%
I AT -20"
SlLlClC ACID DEACTIVATED
BY WASHING WITH LARGE
VOLUME OF WATER
I
ACETON E-SOLU BLE
I
ACETONE-INSOLUBLE
EXTRACT 3X WITH
I HOT ACETONE
ACET~NE-SOLUBLE - COMBINE
I REMOVE ACETONE
ACET~NE-SOLUBLE RESIDUE
5.6%
I TREAT 3X WITH METHANOL
2 COOL TO -65"
I
METI-! ANOL-INSOLUBLE
METHANOL-SOLUBLE
I
EXTRACT 2X WITH
METHANOL
I REMOVE METHANOL
METHANOL-SOLUBLE RESIDUE
1.1%
MET J ANOL-SOLUBLE- COMBINE
(270 rig)
S E PA RAT1 0 N
METHYL ALCOHOL-SOLUBLE RESIDUE
(270 yk-1 I WITH FLORlSlL
ACETONE-METHYL ALCOHOL
1.1%
(0.08 Y k ! )
FIG. 9. Procedures used by Banner el al. (1963b) for extraction and separationof ciguatera
toxin. Yields expressed as percentages of original dry weight. Lethality shown in
parenthesis as amount per gram in mice injected intrapcritoneally.