r~~)~)r.oxirn;rt,c.ly :I y ' w s ago irti(1 h s siiicc. c*otrxist,c.iitIy c ( i v ( S i i siii isfiid,ory
rexults ( D a v i s and IJkc.lrx, 1!)61).
The culture apparatus consists of sixteen 5-gal Pyrex carboys as
growth chambers (Fig. 2 2 ) . Vigorous agitation, by bubbling a mixture
of air and CO, through the cultures, keeps the contents of the chambers
thoroughly mixed. This prevents stratification and helps to expose
all cells to cqual periods of strong illumination. The carboys are
immersed to a depth of 3 or 4 in in a water bath kept at a desired
temperature, usually 19" f 1°C. About 3 liter of the algal culture from
each growth chamber are harvested each day, yielding about 1.5 ml
of packed wet cells. The present system, thus, produces daily approximately 50 liter of algal suspension, averaging 0.5 ml of packed cells per
liter.
A volume of sea water, nutrient salts and antibiotics, equal to the
volume of culture drawn off, is added daily to each growth chamber.
The sea water used is first passed through Orlon filters, previously
described, to remove larger particles. Nutrient salts are then added
and this solution is forced through a ceramic bacteriological filter
(Selas No. FP-128-03, maximum pore size 0.6 p ) into the growth
chambers. Recently, wc have been adding to the sea water and nutrient
salts, prior to final filtration, O-002yo Acronize (approximately loyo
chlorotetracycline) to reduce bacterial growth on the ceramic filters.
This concentration of Acronize does not interfere with algal growth
and helps prolong the life of the cultures.
Although Chlorella sp. and a number of other algae will grow on a
media made from 5-3-5 or 6-3-6 fertilizers, a more elaborate media is
needed for our bacteria-free cultures. Since the requirements of each
of the more than eighty species of marine algae maintained at our
laboratory have not been determined, we use the following as '' universal " media, although it is recognized that the necessity for the various
ingredients has not been ascertained. Two stock solutions of nutrient
salts are prepared and 1 ml of each is used per liter of sea water.
Solution A
Dissolve in 1 liter of distilled water
NaH,PO,
20.0 g
Thiamine H C1
0.2 g
Biotin
0401 g
BIZ
0401 g
Pyradoxine HCl
0.1 g
Calcium pantothenate
0.2 g
rexults ( D a v i s and IJkc.lrx, 1!)61).
The culture apparatus consists of sixteen 5-gal Pyrex carboys as
growth chambers (Fig. 2 2 ) . Vigorous agitation, by bubbling a mixture
of air and CO, through the cultures, keeps the contents of the chambers
thoroughly mixed. This prevents stratification and helps to expose
all cells to cqual periods of strong illumination. The carboys are
immersed to a depth of 3 or 4 in in a water bath kept at a desired
temperature, usually 19" f 1°C. About 3 liter of the algal culture from
each growth chamber are harvested each day, yielding about 1.5 ml
of packed wet cells. The present system, thus, produces daily approximately 50 liter of algal suspension, averaging 0.5 ml of packed cells per
liter.
A volume of sea water, nutrient salts and antibiotics, equal to the
volume of culture drawn off, is added daily to each growth chamber.
The sea water used is first passed through Orlon filters, previously
described, to remove larger particles. Nutrient salts are then added
and this solution is forced through a ceramic bacteriological filter
(Selas No. FP-128-03, maximum pore size 0.6 p ) into the growth
chambers. Recently, wc have been adding to the sea water and nutrient
salts, prior to final filtration, O-002yo Acronize (approximately loyo
chlorotetracycline) to reduce bacterial growth on the ceramic filters.
This concentration of Acronize does not interfere with algal growth
and helps prolong the life of the cultures.
Although Chlorella sp. and a number of other algae will grow on a
media made from 5-3-5 or 6-3-6 fertilizers, a more elaborate media is
needed for our bacteria-free cultures. Since the requirements of each
of the more than eighty species of marine algae maintained at our
laboratory have not been determined, we use the following as '' universal " media, although it is recognized that the necessity for the various
ingredients has not been ascertained. Two stock solutions of nutrient
salts are prepared and 1 ml of each is used per liter of sea water.
Solution A
Dissolve in 1 liter of distilled water
NaH,PO,
20.0 g
Thiamine H C1
0.2 g
Biotin
0401 g
BIZ
0401 g
Pyradoxine HCl
0.1 g
Calcium pantothenate
0.2 g