TABLE II
COMPARISON OF CHARACTERISTICS OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM DIFFERENT SOURCES
Separation of
Highest
Coenzyme
Fold
6-PGA, G-6-P
specific
Optimum
Substrate
Source
specificity
purification
dehydrogenases
activitya
pH
Km
References
Yeast
TPN
45
30% G-6-P dehyd.
15.6
7.4
5 X 10- 5
86
Leuconostoc
sp.
DPN
16
2.0
7.8
7.8 X 10- 5
57
Escherichia coli
TPN
4 % G-6-P dehyd.
2.7
6.6-7.7
3 X 10- 5
52
Acetobacter suboxydans
DPN,TPN
Complete
50
8.0
2.3 X 10- 4
(DPN)
66
1.3 X 10- 3
(TPN)
a
One unit
= amount causing 1.0 optical density change at 340 mJL
under the conditions specified. Specific activity
= units per minute per milligram protein.
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