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One unit
= amount causing 1.0 optical density change at 340 mj.L,
under the conditions listed in the references. Specific activity
= units per minute per milligram
protein.
TABLE I
COMPARISON OF CHARACTERISTICS OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE FROM DIFFERENT SOURCES
Yeast
n-Glucose-6-P04 TPN, Mg or Mn n-a-Gluconalactone-6-P04 600-900
Escherichia coli
n-Glucose-6-P04 TPN, Mg
n-a-Gluconalactone-6-P04
11
Leuconostoc
sp.
n-Glucose-6-P04 TPN, DPN
n-a-Gluconalactone-6-P04
12
Acetobacter suboxydans
n-Glucose-6-P04 TPN, DPN
n-a-Gluconalactone-6-P04
100
n-Glucose-6-P04 TPN
n-Glucose-6-P04 TPN
n-Glucose-6-P04 TPN, DPN
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References
Substrate
Km
8.5
2.0-6.9 X 10- 5
79-83
7.7-8.6
3 X 10- 4
52
7.8
5.3 X 10- 4
56,57
8.0
3.9 X 10- 4
66
(DPN)
69
8.1-8.6
1.
7 X 10- 4
75
63
5 X 10- 5
20
20
20
23
Optimum
pH
36
20
11
86
7
39
100
420
Highest
specific
activitya
Separation
of G-6-P,
6-PGA
dehydrogenase
Complete
Complete
Complete
2% 6-PGA dehyd.
Not stated
Complete
Complete
(crystalline)
Fold
purification
Product
n-a-Gluconalactone-6-P04
n-a-Gluconalactone-6-P04
60
n-a-Gluconalactone-6-P04
25
10,000
Coenzyme
specificity
TPN
TPN
TPN
TPN
Substrate
Source
Neurospora crassa
Aspergillus niger
Azotobacter
dnelandii
Liver
Rat
Horse
Kidney (rat)
Mammary tissue