Figures 13-19.
Organ initiation in organ and tissue culture.
FIG. 13. Isolated root segment of Convolvulus arvensis L. in culture, showing
polar formation of organs and inhibited development of initiated buds (on proximal
end at left) and a root primordium (on distal end at right). X 3.5. (Torrey, unpublished results, 1958.)
FIG. 14. Callus tissue of root of Pisum sativum L., showing partially organized
structures at callus surface which develop into root structures when nutrient conditions are changed. X 5. (Torrey, unpublished data, 1965.)
FIG. 15. Cell colony derived from cell suspension of Convolvulus root callus, cultured on synthetic medium containing a-naphthaleneacetic acid, kinetin, and inositol, showing numerous buds initiated in the callus. X 5. (From Earle and Torrey,
1965b.)
FIG. 16. Transverse section of Convolvulus root segment in culture, cut near the
proximal end, showing a bud primordium 5 days after initiation. Note position
opposite the primary xylem. X 235. (From Bonnett, 1964.)
FIG. 17. Transverse section of Convolvulus root segment in culture, cut near the
distal end, showing a root primordium 60 hr after initiation. Note position of primary xylem, X 245. (From Bonne tt, 1964.)
FIG. 18. Section of a cell colony of Convolvulus root callus derived from cell
suspension grown on a medium containing a-naphthaleneacetic acid and kinetin
(see Fig. 15), showing an organized shoot apex with a leaf primordium. χ 80. (From
Earle and Torrey, 1965b.)
FIG. 19. Section of a callus tissue derived from pea root tissue cultured on a
complex medium (see Fig. 14), showing a partially organized root primordium
from which an organized root can develop under proper stimulus. X 66. (Torrey,
unpublished data, 1965.)
73
Organ initiation in organ and tissue culture.
FIG. 13. Isolated root segment of Convolvulus arvensis L. in culture, showing
polar formation of organs and inhibited development of initiated buds (on proximal
end at left) and a root primordium (on distal end at right). X 3.5. (Torrey, unpublished results, 1958.)
FIG. 14. Callus tissue of root of Pisum sativum L., showing partially organized
structures at callus surface which develop into root structures when nutrient conditions are changed. X 5. (Torrey, unpublished data, 1965.)
FIG. 15. Cell colony derived from cell suspension of Convolvulus root callus, cultured on synthetic medium containing a-naphthaleneacetic acid, kinetin, and inositol, showing numerous buds initiated in the callus. X 5. (From Earle and Torrey,
1965b.)
FIG. 16. Transverse section of Convolvulus root segment in culture, cut near the
proximal end, showing a bud primordium 5 days after initiation. Note position
opposite the primary xylem. X 235. (From Bonnett, 1964.)
FIG. 17. Transverse section of Convolvulus root segment in culture, cut near the
distal end, showing a root primordium 60 hr after initiation. Note position of primary xylem, X 245. (From Bonne tt, 1964.)
FIG. 18. Section of a cell colony of Convolvulus root callus derived from cell
suspension grown on a medium containing a-naphthaleneacetic acid and kinetin
(see Fig. 15), showing an organized shoot apex with a leaf primordium. χ 80. (From
Earle and Torrey, 1965b.)
FIG. 19. Section of a callus tissue derived from pea root tissue cultured on a
complex medium (see Fig. 14), showing a partially organized root primordium
from which an organized root can develop under proper stimulus. X 66. (Torrey,
unpublished data, 1965.)
73