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sections through the fore-limb buds of mole embryos, at the stage of the growing digital buds
(a) Alkahnephosphatase
(v. Kossa-Barger's technique). Enzyme present in the perichondrial tissue surrounding the
precartilages, absent in the precartilages themselves, (b) Polysaccharides (McManus' technique). The process of
chondrincation as revealed by the glycogen in the precartilages, has reached the first phalanx of the three median
digital buds. The arrows indicate glycogen in the distal mesoderm of the digital buds I I and I I I c 1 2 3 4—central
precartilages; Ca 1, 2, 3, 4, 5 - d i s t a l precartilages of the carpus; Cub.-cubitus
precartilage; m 1, 2, 3, 4 5—metapodial precartilages; tfa.-radius precartilage; r - r a d i a l e precartilage; ^ - u l n a r e pre-cartilage; 1-first phalanx
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