780
S U B J E C T I N D E X
Spinal cord,
in aggregates, 506, 510
and cortisone, 607
culture of, 3
development of, 535
differentiation of, 545-6, 548
extracts of, and induction, 481
induction of, 546, 5 5 0 - 1 , 557-8
as inductive agent, 1 5 5 , 4 8 0 - 1 , 5 0 6 , 5 1 0
Spindle (mitotic), 208-13
radiation of, 215
sex hormones and, 616
Spino-caudal induction, 547
b y embryo extract, 552
H e L a cells and, 547, 550
inactivation of, b y pepsin etc., 552
protein component in, 552
Spleen,
cortisone and growth of, 607
extract of, 573
oestrogens and, 616
Sponge,
calcareous, 160-1
methods o f organ culture, 76-7
re-aggregation o f cells of, 490
Spratt's medium,
for avian blastoderms, 554
Spreading factors, 382-3
Squash technique,
for chromosomes, 402
Stabilization (of differentiation), 467
covert, 468
Stainless steel,
for organ cultures, 77
Stereocilia, 158
Stereo tropism, 187
Steroids,
and adrenal cortex, 604
in cell surface, 153, 158
as inducing agents, 543
and ionic balance, 26, 167
and mammary gland, 634
not required b y cell lines, 284
unsaturated, and abnormal mitoses,
686
Stilboestrol (see also Diethylstilboestrol)
and alkaline phosphatase, 627
and kidney tumours, 633
and prostatic carcinoma, 630
Stomach,
epithelial outgrowth from, 46
perfusion methods for, 80
Strain-L ( N C T C . 929),
adaption to m e d i u m N C T C . 109,121,
123
amino-acid requirements, 114, 331
and attachment to glass, 105
behaviour o f daughter cells of, 66
cell cycle of, 222, 422
clone of, 47
development o f malignancy in, 66, 168
D N A synthesis in, 322, 324, 332-3
energy for synthesis of, 241-3
and j8-galactosidase, 264
hydrocortisone and, 607
and inositol, 289
keto-acids and, 125
p H and metabolism of, 258, 266
in protein free-medium, 50, 292
protein synthesis in, 333
radiation and nuclear phosphorylation
in, 344
respiration of, 252, 259
R N A synthesis in, 326, 329
sources o f carbohydrate for, 2 4 5 - 6
suspension cultures, o f 11, 153, 155
in synthetic media, 11, 109-10, 153,
155
vitamins etc., and, 115-16
Strain N C T C . 2071, 116
Streptomycin,
and growth o f fibroblasts, 117
use of, 21
Striations,
cortisone and, 614
as diagnostic o f muscle differentiation,
465
Stripping film,
in autoradiography, 420
Strontium,
and cell aggregation, 500
Stroma (see also Matrix),
and epithelial differentiation, 633
of mammary gland, 634-5
Submetacentric (chromosomes), 403
Succinic dehydrogenase,
in bone, 648
in cultured cells, 250, 475
malonate and, 572
Sucrose,
density gradient of, 45
and neuralization, 543
and stability o f retinal rods, 63
S U B J E C T I N D E X
Spinal cord,
in aggregates, 506, 510
and cortisone, 607
culture of, 3
development of, 535
differentiation of, 545-6, 548
extracts of, and induction, 481
induction of, 546, 5 5 0 - 1 , 557-8
as inductive agent, 1 5 5 , 4 8 0 - 1 , 5 0 6 , 5 1 0
Spindle (mitotic), 208-13
radiation of, 215
sex hormones and, 616
Spino-caudal induction, 547
b y embryo extract, 552
H e L a cells and, 547, 550
inactivation of, b y pepsin etc., 552
protein component in, 552
Spleen,
cortisone and growth of, 607
extract of, 573
oestrogens and, 616
Sponge,
calcareous, 160-1
methods o f organ culture, 76-7
re-aggregation o f cells of, 490
Spratt's medium,
for avian blastoderms, 554
Spreading factors, 382-3
Squash technique,
for chromosomes, 402
Stabilization (of differentiation), 467
covert, 468
Stainless steel,
for organ cultures, 77
Stereocilia, 158
Stereo tropism, 187
Steroids,
and adrenal cortex, 604
in cell surface, 153, 158
as inducing agents, 543
and ionic balance, 26, 167
and mammary gland, 634
not required b y cell lines, 284
unsaturated, and abnormal mitoses,
686
Stilboestrol (see also Diethylstilboestrol)
and alkaline phosphatase, 627
and kidney tumours, 633
and prostatic carcinoma, 630
Stomach,
epithelial outgrowth from, 46
perfusion methods for, 80
Strain-L ( N C T C . 929),
adaption to m e d i u m N C T C . 109,121,
123
amino-acid requirements, 114, 331
and attachment to glass, 105
behaviour o f daughter cells of, 66
cell cycle of, 222, 422
clone of, 47
development o f malignancy in, 66, 168
D N A synthesis in, 322, 324, 332-3
energy for synthesis of, 241-3
and j8-galactosidase, 264
hydrocortisone and, 607
and inositol, 289
keto-acids and, 125
p H and metabolism of, 258, 266
in protein free-medium, 50, 292
protein synthesis in, 333
radiation and nuclear phosphorylation
in, 344
respiration of, 252, 259
R N A synthesis in, 326, 329
sources o f carbohydrate for, 2 4 5 - 6
suspension cultures, o f 11, 153, 155
in synthetic media, 11, 109-10, 153,
155
vitamins etc., and, 115-16
Strain N C T C . 2071, 116
Streptomycin,
and growth o f fibroblasts, 117
use of, 21
Striations,
cortisone and, 614
as diagnostic o f muscle differentiation,
465
Stripping film,
in autoradiography, 420
Strontium,
and cell aggregation, 500
Stroma (see also Matrix),
and epithelial differentiation, 633
of mammary gland, 634-5
Submetacentric (chromosomes), 403
Succinic dehydrogenase,
in bone, 648
in cultured cells, 250, 475
malonate and, 572
Sucrose,
density gradient of, 45
and neuralization, 543
and stability o f retinal rods, 63