744
S U B J E C T I N D E X
Egg white—continued
extract and culture o f ova, 576
in m e d i u m for blastoderms, 553
and neuralization o f ectoderm, 543
Eh,
and culture o f lens, 80
Elastase,
and cell dissociation, 57, 515
and uptake o f sulphate, 379
Elastomucase, 57
Electrical stimuli,
and cell movement, 190-1
Electrolyte balance, see Ionic equilibrium
Electron-microscope,
and epidermis, 51
uses in tissue culture, 12, 37
Electron-transfer systems, 255-7
Electrophoresis,
of cells, 46, 164
of glycoproteins, 365
of inducing agent, 481, 552
of mucopolysaccharides, 362
of peptones, 122
Embden-Meyerhof pathway,
enzymes of, 248
Embryo,
cell aggregates of, 510
-chimaera, 581
culture o f whole, 531, 5 7 8 - 9
formed from two ova, 580
Embryo extract,
ascorbic acid and, 684, 688
and bone growth, with insulin, 597-8
and cell movement, 183
contamination o f cultures by, 34
of different ages, 470
and differentiation, 470
enzymes in, 247, 310, 366
first use of, 3
and growth o f blastoderms, 555, 578
and growth o f epitheliocytes, 157
and growth o f fibroblasts, 157, 165
growth rate, and melanogenesis, 644
in hanging-drop cultures, 31
homologous or heterologous, 31, 35,
579
hyaluronic acid in, 366, 383
as inducing agent, 542, 544, 552
and mucopolysaccharide production,
373
nucleic acids, 3 4 - 5 , 256
in organ cultures, 7 4 - 5 , 470, 597
properties of, 34, 256
proteolysis in, 310
and re-aggregation, 499
and reducing activity, 567
as supplement to defined media, 470
Embryogenesis, 6, 5 3 2 - 8 4
Embryological watch-glass,
for organ culture, 73, 75
Encapsulation,
o f organ cultures, 76
Endocrinology,
organ culture and the study of, 6, 14
Endoderm, 537
and connective tissue support, 538
determination of, 538
morphogenetic movements in, 560,563
and orientation, 556, 560
separation from mesectoderm, 556
Endometrium,
ionic balance in, 167
oestradiol and, 306
oestrogens and, 627
Endoplasmic reticulum, 158, 161
Endoreduplication,
in mouse tumour, 423
Endothelial cells,
centrospheres in, 165
contacts between, 163
in cultures o f heart, 608
D O G and, 608
and epithelial cells, 165
nature of, 165
in outgrowth o f thymus, 143
and production o f chondroitin sulphate, 372
as source o f mechanocytes, 147
Energy,
for differentiation, 244
for synthesis o f a single cell, 241
Energy metabolism, 2 3 9 - 6 8
connected with special metabolic states,
239
and environment, 240
of growing cells, 2 4 0 - 4
and mechanical work, 240
Enolase,
and glycolysis o f heart, 571
Environment
cellular, 10-11, 21, 36, 40
Environmental factors,
and cell behaviour, 144, 150-3, 155
and cell movement, 179-81, 187
S U B J E C T I N D E X
Egg white—continued
extract and culture o f ova, 576
in m e d i u m for blastoderms, 553
and neuralization o f ectoderm, 543
Eh,
and culture o f lens, 80
Elastase,
and cell dissociation, 57, 515
and uptake o f sulphate, 379
Elastomucase, 57
Electrical stimuli,
and cell movement, 190-1
Electrolyte balance, see Ionic equilibrium
Electron-microscope,
and epidermis, 51
uses in tissue culture, 12, 37
Electron-transfer systems, 255-7
Electrophoresis,
of cells, 46, 164
of glycoproteins, 365
of inducing agent, 481, 552
of mucopolysaccharides, 362
of peptones, 122
Embden-Meyerhof pathway,
enzymes of, 248
Embryo,
cell aggregates of, 510
-chimaera, 581
culture o f whole, 531, 5 7 8 - 9
formed from two ova, 580
Embryo extract,
ascorbic acid and, 684, 688
and bone growth, with insulin, 597-8
and cell movement, 183
contamination o f cultures by, 34
of different ages, 470
and differentiation, 470
enzymes in, 247, 310, 366
first use of, 3
and growth o f blastoderms, 555, 578
and growth o f epitheliocytes, 157
and growth o f fibroblasts, 157, 165
growth rate, and melanogenesis, 644
in hanging-drop cultures, 31
homologous or heterologous, 31, 35,
579
hyaluronic acid in, 366, 383
as inducing agent, 542, 544, 552
and mucopolysaccharide production,
373
nucleic acids, 3 4 - 5 , 256
in organ cultures, 7 4 - 5 , 470, 597
properties of, 34, 256
proteolysis in, 310
and re-aggregation, 499
and reducing activity, 567
as supplement to defined media, 470
Embryogenesis, 6, 5 3 2 - 8 4
Embryological watch-glass,
for organ culture, 73, 75
Encapsulation,
o f organ cultures, 76
Endocrinology,
organ culture and the study of, 6, 14
Endoderm, 537
and connective tissue support, 538
determination of, 538
morphogenetic movements in, 560,563
and orientation, 556, 560
separation from mesectoderm, 556
Endometrium,
ionic balance in, 167
oestradiol and, 306
oestrogens and, 627
Endoplasmic reticulum, 158, 161
Endoreduplication,
in mouse tumour, 423
Endothelial cells,
centrospheres in, 165
contacts between, 163
in cultures o f heart, 608
D O G and, 608
and epithelial cells, 165
nature of, 165
in outgrowth o f thymus, 143
and production o f chondroitin sulphate, 372
as source o f mechanocytes, 147
Energy,
for differentiation, 244
for synthesis o f a single cell, 241
Energy metabolism, 2 3 9 - 6 8
connected with special metabolic states,
239
and environment, 240
of growing cells, 2 4 0 - 4
and mechanical work, 240
Enolase,
and glycolysis o f heart, 571
Environment
cellular, 10-11, 21, 36, 40
Environmental factors,
and cell behaviour, 144, 150-3, 155
and cell movement, 179-81, 187