S U B J E C T I N D E X
737
Cholesterol—continued
and cloning o f H e L a cells, 684
on glass, and cell activity, 3 1 - 2 , 63
Cholic acid,
as supplement to synthetic medium, 122
Choline,
required b y cell lines, 2 8 1 - 2
in synthetic media, 115-16, 280
Chondriome, see Mitochondria
Chondroblasts,
in aggregates, 507, 522
behaviour of, 4 7 6 - 7 , 597
and chondroitin sulphate-A, 372
corticosteroids and proliferation of,
612
hypertrophy of, 614, 6 4 1 - 2 , 647
and inductive action on myoblasts, 506
insulin and, 5 9 7 - 9
scleral and skeletal, 507
Chondrocytes,
in aggregates, 507
behaviour of, 4 7 6 - 7
and formation o f chondroitin sulphate,
515
generation time of, 476
hydrolytic enzymes of, 6 7 8 - 9
lysosomes of, 309, 679
in monolayers, 515
Chondrogenesis,
factor for, 481
from fresh and aged cell cultures,
476-7
related to serum and embryo juice, 470
Chondrogenic mesenchyme, 506
and mesonephric differentiation, 472
Chondroitin
combination with sulphate, 369, 385
properties and distribution of, 356
Chondroitin sulphate,
and calcification, 372
corticosteroids and, 385, 612, 615
endothelial cells and, 372
formation b y chondrocytes, 515
hydrolysis of, 364
insulin and, 601
liberation of, 381
macromolecular structure of, 367
metachromasia of, 359, 381
and mucin clot, 367
in organ cultures o f cartilage, 371
and PAS-staining, 371
as polyelectrolytes, 364
BB
properties of, 356-7
turnover of, 380
Chondroitin sulphate-A,
in cartilage, 355
identified b y infra-red analysis, 364
produced b y chondroblasts, 372
produced b y fibroblasts, 370
properties of, 356
separation of, 361-2
Chondroitin sulphate-B
cetylpyridinium bromide and, 361
hyaluronidase and, 361
iduronic acid in, 363
produced b y fibroblasts in culture, 370
properties and distribution of, 356
and skin cultures, 369-70
Chondroitin sulphate-C,
identification b y infra-red
spectroscopy, 364
produced in tissue cultures, 369-70
properties and distribution of, 356—7
Chondroitin sulphate-D,
in sharks, 357
Chondrosin, 364
Chordo-mesoderm, 534
as gradient field, 535
potentialities of, 538
as inducing agent, 541, 556
Chorio-allantoic membrane,
grafts on, 4 7 1 - 2 , 578
Choroid plexus,
cell types in, 160
Chromatids,
appearance of, in mitosis, 415
behaviour of, 4 2 5 - 8
Chromatin (see also Heterochromatin),
cytidine incorporation into, 327
D N A protein complex in, 342
testosterone and structure of, 624
thymidine incorporation into, 325
Chromatography, 12
and amino-acid estimation, 3 0 3 - 4
of glycoproteins, 365
of inducing agent, 481
and mucopolysaccharides, 361-3
Chromatophores,
induced in ectoderm, 544
Chromic acid,
for glassware, 22
Chromocenters,
o f cells in vitro, 4 4 3 - 4
and centromeres, 431
737
Cholesterol—continued
and cloning o f H e L a cells, 684
on glass, and cell activity, 3 1 - 2 , 63
Cholic acid,
as supplement to synthetic medium, 122
Choline,
required b y cell lines, 2 8 1 - 2
in synthetic media, 115-16, 280
Chondriome, see Mitochondria
Chondroblasts,
in aggregates, 507, 522
behaviour of, 4 7 6 - 7 , 597
and chondroitin sulphate-A, 372
corticosteroids and proliferation of,
612
hypertrophy of, 614, 6 4 1 - 2 , 647
and inductive action on myoblasts, 506
insulin and, 5 9 7 - 9
scleral and skeletal, 507
Chondrocytes,
in aggregates, 507
behaviour of, 4 7 6 - 7
and formation o f chondroitin sulphate,
515
generation time of, 476
hydrolytic enzymes of, 6 7 8 - 9
lysosomes of, 309, 679
in monolayers, 515
Chondrogenesis,
factor for, 481
from fresh and aged cell cultures,
476-7
related to serum and embryo juice, 470
Chondrogenic mesenchyme, 506
and mesonephric differentiation, 472
Chondroitin
combination with sulphate, 369, 385
properties and distribution of, 356
Chondroitin sulphate,
and calcification, 372
corticosteroids and, 385, 612, 615
endothelial cells and, 372
formation b y chondrocytes, 515
hydrolysis of, 364
insulin and, 601
liberation of, 381
macromolecular structure of, 367
metachromasia of, 359, 381
and mucin clot, 367
in organ cultures o f cartilage, 371
and PAS-staining, 371
as polyelectrolytes, 364
BB
properties of, 356-7
turnover of, 380
Chondroitin sulphate-A,
in cartilage, 355
identified b y infra-red analysis, 364
produced b y chondroblasts, 372
produced b y fibroblasts, 370
properties of, 356
separation of, 361-2
Chondroitin sulphate-B
cetylpyridinium bromide and, 361
hyaluronidase and, 361
iduronic acid in, 363
produced b y fibroblasts in culture, 370
properties and distribution of, 356
and skin cultures, 369-70
Chondroitin sulphate-C,
identification b y infra-red
spectroscopy, 364
produced in tissue cultures, 369-70
properties and distribution of, 356—7
Chondroitin sulphate-D,
in sharks, 357
Chondrosin, 364
Chordo-mesoderm, 534
as gradient field, 535
potentialities of, 538
as inducing agent, 541, 556
Chorio-allantoic membrane,
grafts on, 4 7 1 - 2 , 578
Choroid plexus,
cell types in, 160
Chromatids,
appearance of, in mitosis, 415
behaviour of, 4 2 5 - 8
Chromatin (see also Heterochromatin),
cytidine incorporation into, 327
D N A protein complex in, 342
testosterone and structure of, 624
thymidine incorporation into, 325
Chromatography, 12
and amino-acid estimation, 3 0 3 - 4
of glycoproteins, 365
of inducing agent, 481
and mucopolysaccharides, 361-3
Chromatophores,
induced in ectoderm, 544
Chromic acid,
for glassware, 22
Chromocenters,
o f cells in vitro, 4 4 3 - 4
and centromeres, 431