2 . M E T H O D S
65
FIG. 6b. Similar colonies of human conjunctiva cells. The epithelial character of the cells
is evident.
(From Puck, Cieciura and Fisher, 1957.)
it is found that practically all the newly introduced cells establish colonies. Further experiments with improved conditions have shown that the
layer of feeder cells can be discarded if certain synthetic media are
combined with serum and embryo extract (Puck, Cieciura and Fisher,
1957). Clones of both epithelial and "fibroblast" cells have been obtained in this way (Fig. 6).
D
65
FIG. 6b. Similar colonies of human conjunctiva cells. The epithelial character of the cells
is evident.
(From Puck, Cieciura and Fisher, 1957.)
it is found that practically all the newly introduced cells establish colonies. Further experiments with improved conditions have shown that the
layer of feeder cells can be discarded if certain synthetic media are
combined with serum and embryo extract (Puck, Cieciura and Fisher,
1957). Clones of both epithelial and "fibroblast" cells have been obtained in this way (Fig. 6).
D