2 .
M E T H O D S
47
2. Mechanical Isolation
The initiation of strains from single cells has not proved to be very
easy; indeed fibroblasts or mechanocytes have turned out to be very
difficult to coax into growth in this way and, for a long time, no one
succeeded in growing such cells from single progenitors.
This failure was probably caused by the difficulty of finding exactly
the right medium for them and Sanford, Earle and Likely (1948) were
FIG. 3. The establishment of clones from single cells in capillary tubes. The figures show the
colonies emerging into the growth medium. (From Perry, Sanford,Evans, Hyatt and Earle, 1957.)
(a) From strain-L mouse fibroblasts.
M E T H O D S
47
2. Mechanical Isolation
The initiation of strains from single cells has not proved to be very
easy; indeed fibroblasts or mechanocytes have turned out to be very
difficult to coax into growth in this way and, for a long time, no one
succeeded in growing such cells from single progenitors.
This failure was probably caused by the difficulty of finding exactly
the right medium for them and Sanford, Earle and Likely (1948) were
FIG. 3. The establishment of clones from single cells in capillary tubes. The figures show the
colonies emerging into the growth medium. (From Perry, Sanford,Evans, Hyatt and Earle, 1957.)
(a) From strain-L mouse fibroblasts.