2 . M E T H O D S
39
•o
(i)
FIG. 2. Slide chambers.
(a) Rose type. Two circular coverslips, spaced by a silicone rubber ring, are clamped between
two steel plates. Hypodermic needles inserted through the rubber allow the introduction of
medium or gas.
(b) Mackaness type. A slide made of Perspex (Lucite) or stainless steel has a central aperture
which is counterbored on both sides to receive two circular coverslips Coverslips are
sealed on with wax. Medium or gas is introduced through two lateral holes which are sealed
by small plugs.
(c) Pulvertaft type, (i) A glass slide with a central pillar of agar. Coverslip sealed round with
Vaseline, (ii) A Perspex (Lucite) slide with a central pillar and a moat. Coverslip sealed on
with wax.
Note: In most cases a gas bubble can be introduced into the chamber. This provides a controlled gas phase and if the slide is rotated the medium is effectively stirred by the bubble.
of the difficulties of the early perfusion chambers which has thus
been overcome is that the whole assembly can be sterilized by dry heat
or in ether, and no toxic substances appear to be liberated into the
medium during the growth period.
In such chambers the cells may be grown directly on the glass,
in a clot of plasma, under Cellophane (Rose, Pomerat, Shindler and
Trunnell, 1958), between Cellophane sheets (Rose, 1962) or between
serum-agar and the upper coverslip, or with various combinations of
these. It is often important for optical reasons to keep the cells in a single
layer with the minimum of distortion by the presence of structures
a
b
c
(ii)
39
•o
(i)
FIG. 2. Slide chambers.
(a) Rose type. Two circular coverslips, spaced by a silicone rubber ring, are clamped between
two steel plates. Hypodermic needles inserted through the rubber allow the introduction of
medium or gas.
(b) Mackaness type. A slide made of Perspex (Lucite) or stainless steel has a central aperture
which is counterbored on both sides to receive two circular coverslips Coverslips are
sealed on with wax. Medium or gas is introduced through two lateral holes which are sealed
by small plugs.
(c) Pulvertaft type, (i) A glass slide with a central pillar of agar. Coverslip sealed round with
Vaseline, (ii) A Perspex (Lucite) slide with a central pillar and a moat. Coverslip sealed on
with wax.
Note: In most cases a gas bubble can be introduced into the chamber. This provides a controlled gas phase and if the slide is rotated the medium is effectively stirred by the bubble.
of the difficulties of the early perfusion chambers which has thus
been overcome is that the whole assembly can be sterilized by dry heat
or in ether, and no toxic substances appear to be liberated into the
medium during the growth period.
In such chambers the cells may be grown directly on the glass,
in a clot of plasma, under Cellophane (Rose, Pomerat, Shindler and
Trunnell, 1958), between Cellophane sheets (Rose, 1962) or between
serum-agar and the upper coverslip, or with various combinations of
these. It is often important for optical reasons to keep the cells in a single
layer with the minimum of distortion by the presence of structures
a
b
c
(ii)