386
J. T . D I N G L E A N D M. W E B B
In this chapter an attempt has been made to emphasize the contributions of Tissue-Culture techniques in the development of mucopolysaccharide physiology. These techniques undoubtedly offer many
advantages, provided they can be used in conjunction with sufficiently
selective and sensitive analytical methods. Thus the development and
adaptation of the latter to the micro-scale of Tissue Culture seems to be
of paramount importance. Given these tools, which as yet are only
partially available, Tissue Culture and in particular organ culture
should yield much fascinating and important information on the role
of the mucopolysaccharides in cell biology.
R E F E R E N C E S
Adams, J. B. (1959a). Biosynthesis o f chondroitin sulphates. Nature, Lond. 184, 274.
Adams, J. B. (1959b). Effect o f chondroitin on the uptake o f radioactive sulphate into
chondroitin sulphate. Biochim. biophys. Acta 32, 559.
Adams, J. B. (1960). Biosynthesis o f chondroitin sulphate. Incorporation o f sulphate
into chondroitin sulphate in embryonic chick cartilage. Biochem. J. 76, 520.
Aminoff, D . (1961). Methods for the quantitative estimation o f yV-acetymeuraminic
acid and their application to hydrolysates o f sialomucoids. Biochem. J. 81, 384.
Anderson, A . J. (1960). T h e presence o f sialic acid in human cartilage. Biochem. J.
76, 53P.
Anderson, A . J . (1961). T h e occurrence o f sialic acid in human cartilage. Biochem. J.
78, 399.
Anderson, A . J . (1962). Some studies on the relationship between sialic acid and the
mucopolysaccharide-protein complexes in human cartilage. Biochem. J. 82, 372.
Antonopoulos, G. A . , Borelius, E., Gardell, S., Hamstrom, B. and Scott, J. E. (1961).
T h e precipitation o f poly anions by long-chain aliphatic a m m o n i u m compounds.
I V . Elution in salt solutions o f mucopolysaccharide-quaternary ammonium
complexes adsorbed on a support. Biochim. biophys. Acta 54, 213.
Antonopoulos, C . A . , Gardell, S., Szirmai, J. A . and D e Tyssonsk, E. R . (1962).
Quantitative determinations o f glycosaminoglycans (mucopolysaccharides) on
the microgram scale. Biochem. J. 83, 3IP.
Balazs, E. A . and V o n Euler, J. (1952). T h e hyaluronidase content o f necrotic tumor
and testis tissue. Cancer Res. 12, 326.
Barker, S. A . , Stacey, M . , Tipper, D . J . and Kirkham, J. A . (1959). Certain m u c o -
proteins containing neuraminic acid. Nature, Lond. 184, 68.
Becker, G. E. and Day, H . G. (1953). Utilisation o f glucosone and the synthesis o f
glucosamine in the rat. J. biol. Chem. 201, 795.
Belcher, R . and Wilson, C. L. (1956). " N e w Methods in Analytical Chemistry."
Chapman and Hall, L o n d o n .
Berenson, G . S. (1958). A c i d mucopolysaccharides o f bovine aorta studied with aid o f
chromatographic procedure for separating sulphated mucopolysaccharides.
Biochim. biophys. Acta 28, 176.
Berenson, G . S., Lumpkin, W . M . and Shipp, D . O . (1958). Study o f the time-course
production o f acid mucopolysaccharides by fibroblasts in a synthetic medium.
Anat. Rec. 132, 585.
Bettelheim-Jevons, F. R . (1958). Protein-carbohydrate complexes. Advanc. Protein
Chem. 13, 35.
J. T . D I N G L E A N D M. W E B B
In this chapter an attempt has been made to emphasize the contributions of Tissue-Culture techniques in the development of mucopolysaccharide physiology. These techniques undoubtedly offer many
advantages, provided they can be used in conjunction with sufficiently
selective and sensitive analytical methods. Thus the development and
adaptation of the latter to the micro-scale of Tissue Culture seems to be
of paramount importance. Given these tools, which as yet are only
partially available, Tissue Culture and in particular organ culture
should yield much fascinating and important information on the role
of the mucopolysaccharides in cell biology.
R E F E R E N C E S
Adams, J. B. (1959a). Biosynthesis o f chondroitin sulphates. Nature, Lond. 184, 274.
Adams, J. B. (1959b). Effect o f chondroitin on the uptake o f radioactive sulphate into
chondroitin sulphate. Biochim. biophys. Acta 32, 559.
Adams, J. B. (1960). Biosynthesis o f chondroitin sulphate. Incorporation o f sulphate
into chondroitin sulphate in embryonic chick cartilage. Biochem. J. 76, 520.
Aminoff, D . (1961). Methods for the quantitative estimation o f yV-acetymeuraminic
acid and their application to hydrolysates o f sialomucoids. Biochem. J. 81, 384.
Anderson, A . J. (1960). T h e presence o f sialic acid in human cartilage. Biochem. J.
76, 53P.
Anderson, A . J . (1961). T h e occurrence o f sialic acid in human cartilage. Biochem. J.
78, 399.
Anderson, A . J . (1962). Some studies on the relationship between sialic acid and the
mucopolysaccharide-protein complexes in human cartilage. Biochem. J. 82, 372.
Antonopoulos, G. A . , Borelius, E., Gardell, S., Hamstrom, B. and Scott, J. E. (1961).
T h e precipitation o f poly anions by long-chain aliphatic a m m o n i u m compounds.
I V . Elution in salt solutions o f mucopolysaccharide-quaternary ammonium
complexes adsorbed on a support. Biochim. biophys. Acta 54, 213.
Antonopoulos, C . A . , Gardell, S., Szirmai, J. A . and D e Tyssonsk, E. R . (1962).
Quantitative determinations o f glycosaminoglycans (mucopolysaccharides) on
the microgram scale. Biochem. J. 83, 3IP.
Balazs, E. A . and V o n Euler, J. (1952). T h e hyaluronidase content o f necrotic tumor
and testis tissue. Cancer Res. 12, 326.
Barker, S. A . , Stacey, M . , Tipper, D . J . and Kirkham, J. A . (1959). Certain m u c o -
proteins containing neuraminic acid. Nature, Lond. 184, 68.
Becker, G. E. and Day, H . G. (1953). Utilisation o f glucosone and the synthesis o f
glucosamine in the rat. J. biol. Chem. 201, 795.
Belcher, R . and Wilson, C. L. (1956). " N e w Methods in Analytical Chemistry."
Chapman and Hall, L o n d o n .
Berenson, G . S. (1958). A c i d mucopolysaccharides o f bovine aorta studied with aid o f
chromatographic procedure for separating sulphated mucopolysaccharides.
Biochim. biophys. Acta 28, 176.
Berenson, G . S., Lumpkin, W . M . and Shipp, D . O . (1958). Study o f the time-course
production o f acid mucopolysaccharides by fibroblasts in a synthetic medium.
Anat. Rec. 132, 585.
Bettelheim-Jevons, F. R . (1958). Protein-carbohydrate complexes. Advanc. Protein
Chem. 13, 35.