374
J. T . D I N G L E A N D M . W E B B
Tissue o f origin
Rate o f mucopolysaccharide
production
^ g / h / 1 0
6 cells
Reference
H u m a n synovial tissue
1-25-10-9*
Castor (1959)
R a t calvaria
1-1-1-6*
Morris (1960)
Embryonic rat dermis
1-5-50
Daniel et al. (1961)
* Calculated from values given for a 24 h period.
ceased later in the feeding period. A change of medium appeared
to trigger this cycle, which was repeated after each additional change
of medium. Alterations in the rate of mucopolysaccharide production,
described by Daniel et al. (1961), paralleled the rate of glucose utilization in the culture medium, and was inversely proportional to the rate
0-06
10-05
0-04
0-03
0-02
H0-0I
0>
o
C7>
E
c
o
o
N
3
CD
(/)
o
o
J=?
o
2 0
3 0
4 0
Culture period (h)
FIG. 1. Production of hyaluronic acid (mucin clot) and glucose utilization by rat dermal
fibroblasts (line 16 S). Results expressed in terms of cell number. • — • , Clot production;
• — • , glucose utilization.
Clot production (/xg/10
5
cells/h)
T a b l e
I I I
Rates o f mucopolysaccharide production in cultures o f
fibroblasts from different sources