336
J . S E E D
_ 60
<
Q
r so
c 4 0
C D
^ 301
20
o
E
o
|
I0|
C D
4 0
30
20
5>> •
• ••
0
40
80
120
160
200
240
Total nuclear dry mass x I0~
12 g
FIG. 4. Ultraviolet absorption (total nucleic acid) and Feulgen stain (DNA) measurements
for individual monkey kidney cell nuclei plotted against the corresponding dry mass values
(from Fig. 2).
There is a high correlation between dry mass and total nucleic acid (r = 0-931) and
between dry mass and D N A content (r = 0-915), with no significant difference between the
two correlation coefficients (t = 0-66, whereas the value to be attained at the P = 0-05 level
is t = 1-98, and at P = 0-5 level t = 0-68). There is no significant non-linearity in either
correlation (F = 1-81, F = 1-24, whereas F = 2-73 is required at the P = 0-05 level).
Total nuclear nucleic acid x 10
1
2
g
Relative amount of Feulgen stain (DNA)