T A B L E
II
Summary o f population-dependent requirements b y cultured mammalian cells for metabolites which
they can in fact synthesize
(Eagle and Piez, 1962a)
Metabolite required
Cell line
Concentration (HIM)
permitting growth at l o w
population densities
Population density in excess o f
which exogenous metabolite
becomes unnecessary*
Serine
Cystine
Homocystine
Glutamine
Pyruvate
Inositol
In m e d i u m containing
cystine
In m e d i u m containing
homocystine as a
cystine precursor
In m e d i u m with n o
homocystine
In m e d i u m containing
homocystine
In m e d i u m lacking
cystine
f H u m a n t
<( M o u s e (P388)
L R a b b i t ( R T 6 )
H u m a n f
H u m a n f
H u m a n f
H u m a n |
H u m a n ( H e L a and
conjunctiva cells
adapted to growth in
glutamic acid)
M o u s e (P388)
Detroit 98C-6B (15)
H u m a n ( H e L a )
0-005-0-02
0-005
0-005
0-04
0-002-0-005
0-002-0-005
0-01
0-01-0-05
(varies with cell population
density and glutamic acid
concentration)
0-1-1
0-1-1
0-001
50-200
150,000
50,000
50-500
200,000-500,000
10,000-60,000
200,000-500,000
> 50,000
(varies with glutamic acid
concentration)
50,000
100f
200,000**
•Permitting growth at half of maximal rate, based on average generation time over period of 8 days.
•fHeLa, Conjunctiva, KB, "Liver".
**Not regularly reproducible.
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