268
J O H N P A U L
decision in this prolonged controversy must be imminent. Observations already published, utilizing material of this kind, seem to indicate
a difference in the glycolytic capacity of normal cells and virus-induced
tumour cells (Ashmore, Weber, Banerjee and Love, 1961).
A D D E N D U M
Since completion of the original manuscript, thanks to the collaboration of Professor M . G. P. Stoker and Dr. I. MacPherson, we have
had an opportunity to compare several clonal strains of BHK (baby
hamster kidney) cells with transformed strains which originated from
them by treatment with polyoma virus. The untransformed lines behave
as tumour cells on inoculation into hamsters. It has been found that
the glycolytic capacity of the transformed cells is invariably higher than
that of the original lines although respiration is similar in both. Thus
Warburg's findings are borne out in relation to glycolysis but not in
relation to respiration in these cells.
R E F E R E N C E S
A b d e l - T a w a b , G . A . , Broda, E. and Kellner, G . (1959). T h e production o f pyruvic
acid, oxaloacetic acid and a-oxoglutaric acid from glucose b y tissue in culture.
Biochem. J. 72, 619.
A d e b o n o j o , F. O . , Bensch, K . G . and King, D . W . (1961). T h e effect o f nitrogen on
the enzymatic pattern o f strain L cells. Cancer Res. 21, 252.
Agol, V . I. (1960a). O n the penetration o f certain sugars into cells in tissue culture.
Dokl. Akad. Nauk. SSSR 130, 659.
Agol, V . I. (1960b). Glucose transport into tissue culture cells. Biochimia 25, 1092.
Agol, V . I. (1960c). Problems o f the specificity o f carbohydrate and respiratory
metabolism in cultures o f tumours. Akad. Nauk. SSSR Usp. sovr. biol. 49, 37.
Agol, V . I., Gershanovitch, V . N . and Etingof, R . N . (1959). Comparative metabolic
characteristics o f normal and tumor cell cultures. Biochimia 24, 101.
A g o l , V . I., Gershanovitch, V . N . and Etingof, R . N . (1960). O n oxidative and
carbohydrate metabolism similarity in cultures o f normal and malignant tissues.
Trudy Inst. Eksp. Med. Akad. Med. Nauk. SSSR, 96.
Allsopp, G. B. and Fell, H . B. (1950). T h e effects o f fluoroacetate on beating embryo
chick heart in tissue culture. Exp. Cell Res. 1, 590.
Ashmore, J., W e b e r , G., Banerjee, G . and Love, W . G. (1961). Glucose metabolism
of tumors induced b y R o u s sarcoma virus. I I . Isotope studies o f alternate pathways. J. nat. Cancer Inst. 27, 863.
Astrup, T . , Ehrensvard, G., Fischer, A . and Ohlenschlager, V . (1947). Protein
metabolism o f tissue cells in vitro'. V I . T h e accessory growth substances. Acta
physiol. scand. 14, 195.
Auerbach, V . H . and Walker, D . L. (1959). Further studies o n the enzymic pattern
o f a cultured cell line from liver. Biochim. biophys. Acta 31, 268.
Bailey, J. M . , Gey, G . O . and G e y , M . K . (1959). Carbohydrate nutrition and
J O H N P A U L
decision in this prolonged controversy must be imminent. Observations already published, utilizing material of this kind, seem to indicate
a difference in the glycolytic capacity of normal cells and virus-induced
tumour cells (Ashmore, Weber, Banerjee and Love, 1961).
A D D E N D U M
Since completion of the original manuscript, thanks to the collaboration of Professor M . G. P. Stoker and Dr. I. MacPherson, we have
had an opportunity to compare several clonal strains of BHK (baby
hamster kidney) cells with transformed strains which originated from
them by treatment with polyoma virus. The untransformed lines behave
as tumour cells on inoculation into hamsters. It has been found that
the glycolytic capacity of the transformed cells is invariably higher than
that of the original lines although respiration is similar in both. Thus
Warburg's findings are borne out in relation to glycolysis but not in
relation to respiration in these cells.
R E F E R E N C E S
A b d e l - T a w a b , G . A . , Broda, E. and Kellner, G . (1959). T h e production o f pyruvic
acid, oxaloacetic acid and a-oxoglutaric acid from glucose b y tissue in culture.
Biochem. J. 72, 619.
A d e b o n o j o , F. O . , Bensch, K . G . and King, D . W . (1961). T h e effect o f nitrogen on
the enzymatic pattern o f strain L cells. Cancer Res. 21, 252.
Agol, V . I. (1960a). O n the penetration o f certain sugars into cells in tissue culture.
Dokl. Akad. Nauk. SSSR 130, 659.
Agol, V . I. (1960b). Glucose transport into tissue culture cells. Biochimia 25, 1092.
Agol, V . I. (1960c). Problems o f the specificity o f carbohydrate and respiratory
metabolism in cultures o f tumours. Akad. Nauk. SSSR Usp. sovr. biol. 49, 37.
Agol, V . I., Gershanovitch, V . N . and Etingof, R . N . (1959). Comparative metabolic
characteristics o f normal and tumor cell cultures. Biochimia 24, 101.
A g o l , V . I., Gershanovitch, V . N . and Etingof, R . N . (1960). O n oxidative and
carbohydrate metabolism similarity in cultures o f normal and malignant tissues.
Trudy Inst. Eksp. Med. Akad. Med. Nauk. SSSR, 96.
Allsopp, G. B. and Fell, H . B. (1950). T h e effects o f fluoroacetate on beating embryo
chick heart in tissue culture. Exp. Cell Res. 1, 590.
Ashmore, J., W e b e r , G., Banerjee, G . and Love, W . G. (1961). Glucose metabolism
of tumors induced b y R o u s sarcoma virus. I I . Isotope studies o f alternate pathways. J. nat. Cancer Inst. 27, 863.
Astrup, T . , Ehrensvard, G., Fischer, A . and Ohlenschlager, V . (1947). Protein
metabolism o f tissue cells in vitro'. V I . T h e accessory growth substances. Acta
physiol. scand. 14, 195.
Auerbach, V . H . and Walker, D . L. (1959). Further studies o n the enzymic pattern
o f a cultured cell line from liver. Biochim. biophys. Acta 31, 268.
Bailey, J. M . , Gey, G . O . and G e y , M . K . (1959). Carbohydrate nutrition and