3. C O N S T R U C T I O N A N D USE OF S Y N T H E T I C M E D I A
133
Billen, D . and Debrunner, G . A . (1960). Continuously propagating cells derived from
normal mouse bone marrow. J. nat. Cancer Inst. 25, 1127.
Brown, H., Sanger, F. and Kitai, R . (1955). Structure o f pig and sheep insulins.
Biochem. J. 60, 556.
Brues, A . M . and Masters, C. M . (1936). Effects o f osmotic pressure on normal and
malignant fibroblasts. Amer. J. Cancer 28, 314.
Bryant, J. C , Schilling, E. L., Earle, W . R . and Evans, V . J . (1960). Massive fluidsuspension cultures o f certain mammalian tissue cells. III. Exploratory supplementation experiments with chemically defined medium N C T C 109. J. nat.
Cancer Inst. 24, 859.
Bryant, J. G , Evans, V . J., Schilling, E. L. and Earle, W . R . (1961). Effect of chemically defined medium N C T C 109 supplemented with methocel and o f silicone
coating the flasks on strain 2071 cells in suspension culture. J. nat. Cancer Inst.
26, 239.
Burrows, M . T . (1926). Studies on the nature o f the growth stimulus in cancer
J. Cancer Res. 10, 239.
Burrows, M . T . and Neymann, C. A . (1917). Studies in the metabolism o f cells
in vitro. I. T h e toxicity o f a-amino acids for embryonic chicken cells. J. exp. Med.
25, 93.
Carrel, A . (1912). O n the permanent life of tissues outside o f the organism. J. exp.
Med. 15, 516.
Carrel, A . and Burrows, M . T . (1911). O n the physicochemical regulation of the
growth o f tissues. T h e effects of dilution of the medium on the growth of the
spleen. J. exp. Med. 13, 562.
Chang, R . S. (1957). Isolation of nutritional variants from conjunctival and H e L a
cells. Proc. Soc. exp. Biol, N.T. 96, 818.
Chang, R . S. (1958). Differences in inositol requirements of several strains o f H e L a ,
conjunctival and amnion cells. Proc. Soc. exp. Biol., N.T. 99, 99.
Chang, R . S., Pennell, R . B., Keller, W . , Wheaton, L. and Liepins, H . (1959).
Macromolecular growth requirements of human cells in continuous culture.
Proc. Soc. exp. Biol, NT. 102, 213.
Chang, R . S., Liepins, H . and Margolish, M . (1961). Carbon dioxide requirements
and nucleic acid metabolism o f H e L a and conjunctival cells. Proc. Soc. exp. Biol,
NT. 106, 149.
Chu, E. H . Y . and Giles, N . H . (1959). H u m a n chromosome complements in normal
somatic cells in culture. Amer. J. hum. Genet. 11, 63.
Drew, A . H . (1923). Cultivation o f tissues and tumours in vitro. Lancet 204, 785.
Drew, A . H . (1927-28). Notes on the cultivation o f tumours in vitro. Arch. exp. Z
e
^~
forsch. 5, 128.
Dubreuil, R . and Pavilanis, V . (1958). Emploi d'un milieu synth^tique simplifie'
pour la culture in vitro de cellules animales et du virus de la poliomyelite. Canad.
J. Microbiol. 4, 543.
Dulbecco, R . and V o g t , M . (1954). Plaque formation and isolation o f pure lines with
poliomyelitis viruses. J. exp. Med. 99, 167.
Eagle, H . (1955a). T h e specific amino acid requirements o f a mammalian cell
(strain L) in tissue culture. J. biol. Chem. 214, 839.
Eagle, H . (1955b). T h e specific amino acid requirements o f a human carcinoma
cell (strain H e L a ) in tissue culture. J. exp. Med. 102, 37.
Eagle, H . (1955c). Nutrition needs o f mammalian cells in tissue culture. Science 122,
501.
Eagle, H . (1955d). T h e minimum vitamin requirements o f the L and H e L a cells
133
Billen, D . and Debrunner, G . A . (1960). Continuously propagating cells derived from
normal mouse bone marrow. J. nat. Cancer Inst. 25, 1127.
Brown, H., Sanger, F. and Kitai, R . (1955). Structure o f pig and sheep insulins.
Biochem. J. 60, 556.
Brues, A . M . and Masters, C. M . (1936). Effects o f osmotic pressure on normal and
malignant fibroblasts. Amer. J. Cancer 28, 314.
Bryant, J. C , Schilling, E. L., Earle, W . R . and Evans, V . J . (1960). Massive fluidsuspension cultures o f certain mammalian tissue cells. III. Exploratory supplementation experiments with chemically defined medium N C T C 109. J. nat.
Cancer Inst. 24, 859.
Bryant, J. G , Evans, V . J., Schilling, E. L. and Earle, W . R . (1961). Effect of chemically defined medium N C T C 109 supplemented with methocel and o f silicone
coating the flasks on strain 2071 cells in suspension culture. J. nat. Cancer Inst.
26, 239.
Burrows, M . T . (1926). Studies on the nature o f the growth stimulus in cancer
J. Cancer Res. 10, 239.
Burrows, M . T . and Neymann, C. A . (1917). Studies in the metabolism o f cells
in vitro. I. T h e toxicity o f a-amino acids for embryonic chicken cells. J. exp. Med.
25, 93.
Carrel, A . (1912). O n the permanent life of tissues outside o f the organism. J. exp.
Med. 15, 516.
Carrel, A . and Burrows, M . T . (1911). O n the physicochemical regulation of the
growth o f tissues. T h e effects of dilution of the medium on the growth of the
spleen. J. exp. Med. 13, 562.
Chang, R . S. (1957). Isolation of nutritional variants from conjunctival and H e L a
cells. Proc. Soc. exp. Biol, N.T. 96, 818.
Chang, R . S. (1958). Differences in inositol requirements of several strains o f H e L a ,
conjunctival and amnion cells. Proc. Soc. exp. Biol., N.T. 99, 99.
Chang, R . S., Pennell, R . B., Keller, W . , Wheaton, L. and Liepins, H . (1959).
Macromolecular growth requirements of human cells in continuous culture.
Proc. Soc. exp. Biol, NT. 102, 213.
Chang, R . S., Liepins, H . and Margolish, M . (1961). Carbon dioxide requirements
and nucleic acid metabolism o f H e L a and conjunctival cells. Proc. Soc. exp. Biol,
NT. 106, 149.
Chu, E. H . Y . and Giles, N . H . (1959). H u m a n chromosome complements in normal
somatic cells in culture. Amer. J. hum. Genet. 11, 63.
Drew, A . H . (1923). Cultivation o f tissues and tumours in vitro. Lancet 204, 785.
Drew, A . H . (1927-28). Notes on the cultivation o f tumours in vitro. Arch. exp. Z
e
^~
forsch. 5, 128.
Dubreuil, R . and Pavilanis, V . (1958). Emploi d'un milieu synth^tique simplifie'
pour la culture in vitro de cellules animales et du virus de la poliomyelite. Canad.
J. Microbiol. 4, 543.
Dulbecco, R . and V o g t , M . (1954). Plaque formation and isolation o f pure lines with
poliomyelitis viruses. J. exp. Med. 99, 167.
Eagle, H . (1955a). T h e specific amino acid requirements o f a mammalian cell
(strain L) in tissue culture. J. biol. Chem. 214, 839.
Eagle, H . (1955b). T h e specific amino acid requirements o f a human carcinoma
cell (strain H e L a ) in tissue culture. J. exp. Med. 102, 37.
Eagle, H . (1955c). Nutrition needs o f mammalian cells in tissue culture. Science 122,
501.
Eagle, H . (1955d). T h e minimum vitamin requirements o f the L and H e L a cells