3. C O N S T R U C T I O N A N D USE OF S Y N T H E T I C M E D I A
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for various cells and tissues, as we now design diets for man, suitable for
infants, for athletes, for pregnant women and for those with kwashiorkor, anaemia or hypertension.
T A B L E II
Some c o m m o n balanced salt solutions used with mammalian cells and tissues
in vitro (mg/1)
NaCl
9000 9000 8000 7000 8000 6800 8000 8000 7400 8000 8000
KC1
420 420 200 370 370 400 400 400 285 400 200
C a C l 2
250 240 200 170 170 200 200 140
100
C a C l 2 . 2 H 2 0
16
16
M g C l 2 . 6 H 2 0
100* 210 210
M g S 0 4
100
M g S 0 4 . 7 H 2 0
70
70
200 200 154 154
N a H 2 P 0 4 . H 2 0
5 0 * *
140
N a 2 H P 0 4
1150
150 150
100 60
290 290
K H 2 P 0 4
30
30
100
60
83 150 200
N a H C O s
300 1000 2270 227 2200 1273 350 1200
0
Glucose
1000 1000 1000 1000 1000 1000 2000 1000 1100
* M g C l 2
Intending users o f these solutions are advised to consult the original papers.
References for Table II:
(1) Bayliss (1924); Locke (1900); Ringer (1880-2); Ringer (1886)
(2) T y r o d e (1910)
(3) G e y and G e y (1936)
(4) Earle (1943)
(5) Hanks (1948)
(6) Hanks and Wallace (1949)
(7) Puck, Cieciura and Robinson (1958)
(8) D u l b e c c o and V o g t (1954)
* * N a 2 H P Q 4
Ringer
Conventional
Ringer- (1)
Locke
Tyrode (2)
Gey and Gey (3)
For tubes
Gey and Gey (3)
For slides
Earle (4)
Hanks (5)
Hanks (6)
Puck (7)
Saline F
Puck (7)
Saline G
Dulbecco (8)