62
A. SARAGEA E T A L .
X. Practical Procedures of Phage Typing in C. diphtheriue
.
A. Principles .
B. Phage typing proeedure .
C. Notation of results
.
D. Preparation of phage stocks .
E. Conclusions .
A. Basic set of typing phages
.
B. Propagation strains
.
C. Phage typing
.
A. Induction of lysogenic phages in shaken cultures
.
B. Induction of lysogenic phages by ultraviolet irradiation.
Special Techniques and Media used in the Bacteriological Diagnosis
of C. diphtheriue
.
XI. Practical Procedures of Phage Typing C. ulceruns and C. owis
XII. Checking Lysogenicity in Corynebucteria .
XIII.
A. Staining of metachromatic granules .
B. Media .
C. Slide agglutination for serological identification .
D. Media used for phage typing and lysogenicity .
XIV. Principles of Epidemiological Methodology in the Prevention and
Control of Diphtheria
.
A. General
.
B. Surveillance and control of antidiphtheria immunity .
C. Scheme of intervention in a diphtheria outbreak
.
D. General recommendations for the organisation of a reference
diphtheria laboratory
.
XV. General Conclusions
.
References
.
151
151
151
152
152
154
154
154
155
155
155
155
156
157
157
157
162
162
163
163
164
165
167
169
169
I. GENERAL CONSIDERATIONS
The object of this Chapter is to discuss the background of the practical
methods applied by the bacteriologist in diagnosing diphtheria in a suspected case and/or in helping the epidemiologist to uncover epidemic
relationships in diphtheria outbreaks or in endemic conditions, rather than
to deal with the theoretical descriptions of taxa., I t will be based on a
complex methodology applied by the authors in controlling diphtheria in
Romania in the course of 20 years. The same system has been successfully
applied by colleagues, who asked the authors for scientific and technical
assistance, in different reference centres of the world.
It seems that few other diseases of man have been as successfully studied
as diphtheria (Davis et al., 1973) and marked progress is continuously
recorded in different theoretical and practical problems concerning this
infection.
A dramatic decline of diphtheria morbidity and mortality has been
recorded during the decades since the introduction of modern prophylactic
A. SARAGEA E T A L .
X. Practical Procedures of Phage Typing in C. diphtheriue
.
A. Principles .
B. Phage typing proeedure .
C. Notation of results
.
D. Preparation of phage stocks .
E. Conclusions .
A. Basic set of typing phages
.
B. Propagation strains
.
C. Phage typing
.
A. Induction of lysogenic phages in shaken cultures
.
B. Induction of lysogenic phages by ultraviolet irradiation.
Special Techniques and Media used in the Bacteriological Diagnosis
of C. diphtheriue
.
XI. Practical Procedures of Phage Typing C. ulceruns and C. owis
XII. Checking Lysogenicity in Corynebucteria .
XIII.
A. Staining of metachromatic granules .
B. Media .
C. Slide agglutination for serological identification .
D. Media used for phage typing and lysogenicity .
XIV. Principles of Epidemiological Methodology in the Prevention and
Control of Diphtheria
.
A. General
.
B. Surveillance and control of antidiphtheria immunity .
C. Scheme of intervention in a diphtheria outbreak
.
D. General recommendations for the organisation of a reference
diphtheria laboratory
.
XV. General Conclusions
.
References
.
151
151
151
152
152
154
154
154
155
155
155
155
156
157
157
157
162
162
163
163
164
165
167
169
169
I. GENERAL CONSIDERATIONS
The object of this Chapter is to discuss the background of the practical
methods applied by the bacteriologist in diagnosing diphtheria in a suspected case and/or in helping the epidemiologist to uncover epidemic
relationships in diphtheria outbreaks or in endemic conditions, rather than
to deal with the theoretical descriptions of taxa., I t will be based on a
complex methodology applied by the authors in controlling diphtheria in
Romania in the course of 20 years. The same system has been successfully
applied by colleagues, who asked the authors for scientific and technical
assistance, in different reference centres of the world.
It seems that few other diseases of man have been as successfully studied
as diphtheria (Davis et al., 1973) and marked progress is continuously
recorded in different theoretical and practical problems concerning this
infection.
A dramatic decline of diphtheria morbidity and mortality has been
recorded during the decades since the introduction of modern prophylactic