111. TYPING OF E. INSIDIOSA
53
not do so. Strauch and Nitzschke (1958) claimed that haemagglutination
activity of strains was dependent upon the composition of their growth
media. It was Dinter’s intention to use haemagglutination as an indicator of
highly immunogenic strains suitable for the production of inactivated
vaccines.
D. Haemadsorption
Stoev (1969) established that Erysipelothrix has the ability to adsorb
erythrocytes and that the reaction can be suppressed by specific immune
antisera thus extending the technique of haemadsorption, already well
known in the virological field, to the study of these micro-organisms.
E. Distribution of Erysipelothrix serogroups
Hubrig (1962) showed that acute Erysipelothrix infections are caused
mainly by strains belonging to the A serogroup. Serogroups B and N are
detected in less acute infections. Erysipelothrix insidiosa isolated from the
tonsils of healthy pigs in the German Democratic Republic belong to
serogroup N.
Murase et al. (1959) found that 22 Erysipelothrix strains isolated from
diseased pigs showing a septic form of infection belonged to serogroup A.
Nineteen strains isolated from pigs showing urticaria1 forms of infection
were distributed as follows; 16 serogroup B, 3 serogroup A. Fifty strains of
E . insidiosa isolated from the tonsils of pigs belonged to different types;
3 to serogroup A, 22 to serogroup B and the rest to serogroups C, D and F.
The group antigens are polysaccharide in nature (Gralheer, 1951; Brill
et al., 1959; Erler, 1968).
I n view of this association of specific serogroups with different forms of
the disease and with non-specific carriers, the development of rapid and
simple methods of typing E. insidiom has assumed some importance in
the study of the epidemiology of this organism. The rest of this Chapter
will be concerned with a comparative study of the methods available and
the results of their application.
11. METHODS FOR T H E TYPING O F E. INSIDIOSA
A. Bacterial strains
Eighty-five strains of E. insidiosa were used to examine the effectiveness
of different typing methods and the relationships between them. A group
of control organisms consisting of previously typed strains were obtained
from the Dessau Institute in the German Democratic Republic and from
other organisations.
53
not do so. Strauch and Nitzschke (1958) claimed that haemagglutination
activity of strains was dependent upon the composition of their growth
media. It was Dinter’s intention to use haemagglutination as an indicator of
highly immunogenic strains suitable for the production of inactivated
vaccines.
D. Haemadsorption
Stoev (1969) established that Erysipelothrix has the ability to adsorb
erythrocytes and that the reaction can be suppressed by specific immune
antisera thus extending the technique of haemadsorption, already well
known in the virological field, to the study of these micro-organisms.
E. Distribution of Erysipelothrix serogroups
Hubrig (1962) showed that acute Erysipelothrix infections are caused
mainly by strains belonging to the A serogroup. Serogroups B and N are
detected in less acute infections. Erysipelothrix insidiosa isolated from the
tonsils of healthy pigs in the German Democratic Republic belong to
serogroup N.
Murase et al. (1959) found that 22 Erysipelothrix strains isolated from
diseased pigs showing a septic form of infection belonged to serogroup A.
Nineteen strains isolated from pigs showing urticaria1 forms of infection
were distributed as follows; 16 serogroup B, 3 serogroup A. Fifty strains of
E . insidiosa isolated from the tonsils of pigs belonged to different types;
3 to serogroup A, 22 to serogroup B and the rest to serogroups C, D and F.
The group antigens are polysaccharide in nature (Gralheer, 1951; Brill
et al., 1959; Erler, 1968).
I n view of this association of specific serogroups with different forms of
the disease and with non-specific carriers, the development of rapid and
simple methods of typing E. insidiom has assumed some importance in
the study of the epidemiology of this organism. The rest of this Chapter
will be concerned with a comparative study of the methods available and
the results of their application.
11. METHODS FOR T H E TYPING O F E. INSIDIOSA
A. Bacterial strains
Eighty-five strains of E. insidiosa were used to examine the effectiveness
of different typing methods and the relationships between them. A group
of control organisms consisting of previously typed strains were obtained
from the Dessau Institute in the German Democratic Republic and from
other organisations.