C H A P T E R 1 1 1
Methods of Typing Erysipelothrix insidiosa
Iv. STOEV
Institute Against Swine Diseases, Vratza, Bulgaria
I. Introduction
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A. Antigenic structure
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B. Bacteriophage .
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C. Haemagglutination
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D. Haemadsorption.
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E. Distribution of Erysipelothrix serogroups
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11. Methods for the Typing of E. insidiosa
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A. Bacterial strains .
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B. Serotyping
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C. Bacteriophage .
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D. Haemadsorption.
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E. Haemagglutination
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111. Results of the Various Typing Procedures
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A. Typing by microprecipitation
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B. Typing by bacteriophage
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C. Typing by haemadsorption and haemagglutination .
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IV. Discussion
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References
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I. INTRODUCTION
A. Antigen structure
Studies of the antigens of Erysipelothrix insidiosa date from an early
stage in the development of microbiology. Watts (1940) was able to
differentiate two groups of Erysipelothrix by using agglutinogens. Some
strains gave rise to antisera which showed cross agglutination with heterologous organisms. This could be avoided by boiling the antigen used for
injections and Watts concluded that the organisms showed thermostable
group specific antigens and some thermolabile antigens of lower specificity.
Atkinson (1941) also established two major groups on a basis of antigenic structure together with a third group of strains showing a variable
pattern of cross reaction. Gledhill (1945) recognised four groups and
DediC (1949, 1950), using the Lancefield method of precipitin detection,