11. SEROTYPING OF I.ISTERI.4 iMONOCYTOGENES AND RELATED SPECIES 35
The formula of the preferred T N A medium is given below:
Preparation of Trypaflavine-Nalidixic-Acid-Agar :
Tryptose Agar (Difco)
41.05
Nalidixic acid
0.04 g
Distilled water
1000.0 ml
After sterilisation (15 min of lZO°C) the medium is cooled to 70°C and
the acridines", dissolved in distilled water, are added to the required
concentration (25,uglml). The medium is mixed thoroughly and poured
into Petri dishes. T h e medium has to be clear and poured in a thin layer
(about 10 to 13 ml per plate) in order to allow optimal evaluation
(Bockemuhl et ul., 1971).
B. Biochemical Properties
virulent L. monocytogenes isolates show this property.
serological studies (see Tables I and 11).
Properties of Listeria--monocytogenes
During the past few years, non-haemolytic Listeriu isolates have been
grown with increasing frequency from faecal and all sorts of environmental
The demonstration of P-haemolysis is of utmost importance since all
The presumptive diagnosis must be confirmed by biochemical and
T A B L E I
Important properties of Listeria
p-haemolysis
catalase
motility
urea
nitrate reduction
Voges-Proskauer reaction
s a 1 i c i n
trehalose
glucose
lactose
mannitol
aesculin
+
+
+
-
+
+
+
+
acid (without gas)
m
0
+
+ = positive reaction
- = negative reaction
@ = variable or slow reaction
"Acriflavin neutral NOKA 565, CASSELLA Farbwerke, Frankfurt (Main)
FRG or Proflavinhemisulfat NOKR 565, CASSELLA Farbwerke, Frankfurt
(Main) FRG.
The formula of the preferred T N A medium is given below:
Preparation of Trypaflavine-Nalidixic-Acid-Agar :
Tryptose Agar (Difco)
41.05
Nalidixic acid
0.04 g
Distilled water
1000.0 ml
After sterilisation (15 min of lZO°C) the medium is cooled to 70°C and
the acridines", dissolved in distilled water, are added to the required
concentration (25,uglml). The medium is mixed thoroughly and poured
into Petri dishes. T h e medium has to be clear and poured in a thin layer
(about 10 to 13 ml per plate) in order to allow optimal evaluation
(Bockemuhl et ul., 1971).
B. Biochemical Properties
virulent L. monocytogenes isolates show this property.
serological studies (see Tables I and 11).
Properties of Listeria--monocytogenes
During the past few years, non-haemolytic Listeriu isolates have been
grown with increasing frequency from faecal and all sorts of environmental
The demonstration of P-haemolysis is of utmost importance since all
The presumptive diagnosis must be confirmed by biochemical and
T A B L E I
Important properties of Listeria
p-haemolysis
catalase
motility
urea
nitrate reduction
Voges-Proskauer reaction
s a 1 i c i n
trehalose
glucose
lactose
mannitol
aesculin
+
+
+
-
+
+
+
+
acid (without gas)
m
0
+
+ = positive reaction
- = negative reaction
@ = variable or slow reaction
"Acriflavin neutral NOKA 565, CASSELLA Farbwerke, Frankfurt (Main)
FRG or Proflavinhemisulfat NOKR 565, CASSELLA Farbwerke, Frankfurt
(Main) FRG.