448
U. ULLMANN
T A B L E 111
Amino-acid analysis of flagellar protein of C. fetus, in g amino-acid
per 100 g protein (Ullmann, 1976)
H antigen
H antigen
Amino-acid
subspecies venerealis
subspecies intestinalis
Asp.
Thr.
Ser.
Glu.
Pro.
Gly.
Ala.
Val.
Met.
Ileu.
Leu.
Tyr.
Phe.
Lys.
His.
NH3
Arg.
cys.
14.06
6.46
5.10
8.93
+
4.92
6.76
0
6.49
1.33
5.67
9.66
2.87
5.10
9.71
2.88
4.82
4.04
13.18
5.22
5.41
10.94
+
5.05
6.58
0
7.03
1.27
6.56
9.25
3.23
6.41
8.55
2.51
2.05
5.51
4. H antigen factors
Using specific H sera from rabbits, agglutination occurs only with the
homologous antigen if flagellar antisera have been absorbed with heterologous antigens. Investigations of ultrasonic break-down products of
flagella with immunoprecipitation and immunoelectrophoresis show two
common antigenic components: a and c and a partially common antigenic
factor bb (Ullmann, 1973, 1976). In C. fetus subsp. fetus, McCoy et al.
(1975) also found three H antigen factors after acid treatment of flagella,
designated a, d, and e (Table IV).
T A B L E I V
Formula of H antigen factors of C. fetus
Subspecies fetus
Subspecies intestinalis
a, c, bbi
a, d, e
a, c, bbz