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E. A. FHEUNDT E?' At..
5. Proteolytic activity (digestion of coagzrlated serum)
Test medium: medium Sd
Heart infusion broth (Difco)
(Sterilise by autoclaving)
Horse serum
Yeast extract (25 yo) (Taylor-Robinson et al., 1963)
Sterile water
Adjust pH to 7.8
The medium is dispensed in 2 ml volumes into screw cap tubes
and heated in a slanted position in flowing steam for 45 min.
C. Media for metabolism inhibition tests
1. Glucose: medium BG
Heart infusion broth (Difco)
Horse serum (unheated)
Yeast extract (25%) (Taylor-Robinson et al., 1963)
Deoxyribonucleic acid (Sigma 0.2% (w/v) solution)
Glucose (50% (w/v) solution)
Phenol red (60 mg/100 ml)
Thallium acetate (1 % (w/v) solution)
Penicillin (20 000 IU ml-l)
Adjust pH to 7.8
2. Arginine: medium BA
Heart Infusion Broth (Difco)
Horse serum (unheated)
Yeast extract (25%) (Taylor-Robinson et al., 1963)
Deoxyribonucleic acid (Sigma, 0.2% (w/v) solution)
L-arginine (30% (w/v) solution)
Phenol red (60 mg/100 ml)
Thallium acetate (1 94 (w/v) solution)
Penicillin (20 000 IU ml-l)
Adjust pH to 7.3
3. Urea: medium SU (see Section A.3) with pH adjusted to 6.8
4. Tetrazolium: medium BT
Heart infusion broth (Difco)
Horse serum (unheated)
Yeast extract
DNA (Sigma, 0.2% (w/v) solution)
2, 3, 5-triphenyl tetrazolium chloride (1 y, (w/v) solution)
Thallium acetate (1 % (w/v) solution)
Penicillin (20 000 IU ml-1)
Adjust p H to 7.8
8 .O in1
30.0 ml
0.8 ml
1.2 ml
90.0 ml
20.0 ml
10.0 ml
1.3 ml
2.6 ml
5.0 ml
1 .O ml
0.25 ml
90.0 ml
20.0 ml
10.0 ml
1.3 ml
4.25 ml
5.0 ml
1 .O ml
0.25 ml
90.0 ml
20.0 ml
10.0 ml
1.2 ml
5.0 ml
1 .O ml
0.25 ml